Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,860,991	C→A	R237S (CGT→AGT)	yidL →	putative DNA‑binding transcriptional regulator YidL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,860,991	0	C	A	100.0%	123.2 / NA	34	R237S (CGT→AGT)	yidL	putative DNA‑binding transcriptional regulator YidL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/12);  total (22/12)

TAGCGAAGCCTGGTTGCGTCGTCTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGCGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGTCGCTGATACGCTCAACTTCT  >  NC_000913/3860918‑3861066                                                                          |                                                                            tAGCGAAGCCTGGTTGCGTCGTCTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTg                                                                        <  2:749794/79‑1 (MQ=255)   gCGAAGCCTGGTTGCGTCGTCTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATc                                                                    <  2:749795/81‑1 (MQ=255)     gAAGCCTGGTTGCGTCGTCTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTg                                                                  >  2:749847/1‑81 (MQ=255)      aaGCCTGGTTGCGTCGTCTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTgg                                                                 <  2:749796/81‑1 (MQ=255)       aGCCTGGTTGCGTCGTCTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGgc                                                                <  1:749797/81‑1 (MQ=255)          cTGGTTGCGTCGTCTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGcgct                                                             <  1:749798/81‑1 (MQ=255)             gTTGCGTCGTCTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGcgctatc                                                          >  1:749850/1‑81 (MQ=255)                gCGTCGTCTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGcgctatcgc                                                        >  1:749876/1‑80 (MQ=255)                  gtcgtcTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGcgctatcgctat                                                     <  1:749799/81‑1 (MQ=255)                  gtcgtcTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGcgctatcgctat                                                     >  1:749840/1‑81 (MQ=255)                  gtcgtcTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGcgctatcgctat                                                     >  2:749838/1‑81 (MQ=255)                  gtcgtcTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGcgctatcgctat                                                     >  1:749900/1‑81 (MQ=255)                   tcgtcTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGcgctatcgctat                                                     >  2:749848/1‑80 (MQ=255)                   tcgtcTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTAtt                                                    <  2:749800/81‑1 (MQ=255)                     gtcTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTa                                                   >  2:749855/1‑80 (MQ=255)                          tttttACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGca                                              <  2:749802/80‑1 (MQ=255)                          tttttACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGc                                               <  1:749801/79‑1 (MQ=255)                             ttACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGcaac                                            >  2:749846/1‑79 (MQ=255)                                  cTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGaaa                                     >  1:749852/1‑81 (MQ=255)                                       ccGGCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCgg                                >  2:749863/1‑81 (MQ=255)                                         ggCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGtt                              >  1:749866/1‑81 (MQ=255)                                         ggCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGtt                              >  2:749842/1‑81 (MQ=255)                                         ggCAAGACGCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGtt                              <  2:749803/81‑1 (MQ=255)                                                 gCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGt                      >  1:749862/1‑81 (MQ=255)                                                 gCCGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGt                      >  1:749872/1‑81 (MQ=255)                                                   cGAAAGAATATTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGTCg                    >  1:749849/1‑81 (MQ=255)                                                          atatTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGTCGCTGATAc             <  2:749805/81‑1 (MQ=255)                                                          atatTACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGTCGCTGATAc             <  2:749804/81‑1 (MQ=255)                                                              tACCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGTCGCTGATACGCt          >  2:749879/1‑80 (MQ=255)                                                               aCCTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGTCGCTGATACGCTc         >  1:749864/1‑80 (MQ=255)                                                                ccTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGTCGCTGATACGCTCaa       >  2:749858/1‑81 (MQ=255)                                                                ccTCGATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGTCGCTGATACGCTCaa       >  2:749868/1‑81 (MQ=255)                                                                    gATGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGTCGCTGATACGCTCAActtc   >  2:749853/1‑81 (MQ=255)                                                                     aTGCGAGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGTCGCTGATACGCTCAActtct  >  2:749851/1‑81 (MQ=255)                                                                          |                                                                            TAGCGAAGCCTGGTTGCGTCGTCTGTTTTTACGCTATACCGGCAAGACGCCGAAAGAATATTACCTCGATGCGCGTCTGGATCTGGCGCTATCGCTATTAAAGCAACAAGGAAACTCGGTTGGCGAAGTCGCTGATACGCTCAACTTCT  >  NC_000913/3860918‑3861066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑