Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,863,817	A→T	intergenic (‑214/‑82)	glvC ← / → yidP	putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,863,817	0	A	T	100.0%	77.7 / NA	22	intergenic (‑214/‑82)	glvC/yidP	putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (10/12);  total (10/12)

TTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAA  >  NC_000913/3863740‑3863887                                                                              |                                                                       ttGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTtaaa                                                                     <  1:750575/81‑1 (MQ=255)   gggCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTtaaaa                                                                    >  1:750601/1‑80 (MQ=255)                  aCGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCaa                                                    >  2:750610/1‑81 (MQ=255)                   cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGtttttt                                                       <  1:750576/77‑1 (MQ=255)                   cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCa                                                     <  2:750577/79‑1 (MQ=255)                    gTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATc                                                  >  2:750615/1‑81 (MQ=255)                    gTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATc                                                  >  1:750613/1‑81 (MQ=255)                     ttttCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCg                                                 <  2:750579/81‑1 (MQ=255)                     ttttCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCg                                                 <  1:750578/81‑1 (MQ=255)                       ttCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGAt                                               >  2:750609/1‑81 (MQ=255)                        tCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGAt                                               <  1:750580/80‑1 (MQ=255)                         cgcgAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGAtt                                              <  2:750581/80‑1 (MQ=255)                          gcgAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGAtt                                              >  1:750623/1‑79 (MQ=255)                                 aGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAAc                                     <  2:750582/81‑1 (MQ=255)                                 aGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAAc                                     >  2:750617/1‑81 (MQ=255)                                             tgAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTgcgc                           <  1:750583/79‑1 (MQ=255)                                             tgAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCa                          <  1:750584/80‑1 (MQ=255)                                               aGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTa                       <  2:750585/81‑1 (MQ=255)                                               aGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTa                       >  2:750616/1‑81 (MQ=255)                                                 cacaGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTAcc                     <  1:750586/81‑1 (MQ=255)                                                            aGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAg          <  1:750587/81‑1 (MQ=255)                                                             gCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAgg         >  2:750632/1‑81 (MQ=255)                                                              cATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAgg         >  2:750625/1‑80 (MQ=255)                                                                       caTCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCaaaaaa  >  2:750627/1‑78 (MQ=255)                                                                              |                                                                       TTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAA  >  NC_000913/3863740‑3863887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑