Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,887,769	A→C	A314A (GCA→GCC)	mnmE →	5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,887,769	0	A	C	100.0%	198.5 / NA	54	A314A (GCA→GCC)	mnmE	5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/29);  total (25/29)

CGCGTGGCAGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATC  >  NC_000913/3887691‑3887844                                                                               |                                                                            cgcgTGGCAGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCcga                                                                           <  1:757176/81‑1 (MQ=255) cgcgTGGCAGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCcga                                                                           <  2:757175/81‑1 (MQ=255)   cgTGGCAGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCgaga                                                                         <  1:757177/81‑1 (MQ=255)   cgTGGCAGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCgaga                                                                         <  2:757178/81‑1 (MQ=255)    gTGGCAGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGAt                                                                        <  1:757179/81‑1 (MQ=255)       gCAGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCt                                                                      <  2:757181/80‑1 (MQ=255)       gCAGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTg                                                                     <  2:757180/81‑1 (MQ=255)       gCAGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTg                                                                     >  1:757252/1‑81 (MQ=255)        cAGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTgg                                                                    >  2:757260/1‑81 (MQ=255)         aGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGc                                                                   >  2:757258/1‑81 (MQ=255)         aGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGc                                                                   <  1:757182/81‑1 (MQ=255)            aaaTTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCgg                                                                <  1:757183/81‑1 (MQ=255)             aaTTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCgg                                                                >  2:757283/1‑80 (MQ=255)                 gAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAAtt                                                            >  1:757285/1‑80 (MQ=255)                      ggCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGc                                                      <  2:757184/81‑1 (MQ=255)                       gCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGcc                                                     <  1:757185/81‑1 (MQ=255)                        ccGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGccc                                                    >  1:757268/1‑81 (MQ=255)                           aCCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTc                                                 <  2:757186/81‑1 (MQ=255)                              gcgTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGc                                              >  1:757272/1‑81 (MQ=255)                              gcgTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGc                                              >  2:757262/1‑81 (MQ=255)                                gTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGc                                              >  2:757256/1‑79 (MQ=255)                                gTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCa                                            <  2:757187/81‑1 (MQ=255)                                   cTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGc                                          >  2:757274/1‑80 (MQ=255)                                      tttATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGaaa                                      >  1:757264/1‑81 (MQ=255)                                      tttATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGaaa                                      <  2:757188/81‑1 (MQ=255)                                      tttATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGaa                                       <  1:757189/80‑1 (MQ=255)                                          tGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTg                                   <  1:757190/80‑1 (MQ=255)                                           ggTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGcc                                 >  2:757263/1‑81 (MQ=255)                                            gTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGcc                                 <  2:757191/80‑1 (MQ=255)                                            gTCGATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCg                                <  2:757192/81‑1 (MQ=255)                                               gATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGAt                              <  2:757193/80‑1 (MQ=255)                                               gATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGAt                              >  2:757257/1‑80 (MQ=255)                                               gATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGAt                              >  2:757294/1‑80 (MQ=255)                                               gATGGCACCACAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATc                             <  1:757194/81‑1 (MQ=255)                                                    caccacAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCgt                        <  2:757195/81‑1 (MQ=255)                                                     accacAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCgtg                       <  1:757196/81‑1 (MQ=255)                                                     accacAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCgtg                       >  1:757287/1‑81 (MQ=255)                                                      ccacAACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCgtgg                      >  1:757267/1‑81 (MQ=255)                                                       cacaACAGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCgtggt                     >  2:757275/1‑81 (MQ=255)                                                          aacaGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTgcg                  >  1:757273/1‑81 (MQ=255)                                                           acaGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTgcg                  >  2:757296/1‑80 (MQ=255)                                                            caGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCa                <  1:757197/81‑1 (MQ=255)                                                             aGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCaa               <  1:757198/81‑1 (MQ=255)                                                             aGACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCaa               >  1:757288/1‑81 (MQ=255)                                                              gACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCaa               >  1:757286/1‑80 (MQ=255)                                                              gACGCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAAt              <  1:757199/81‑1 (MQ=255)                                                                 gCCGTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATa             >  2:757279/1‑79 (MQ=255)                                                                    gTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGcc        >  1:757290/1‑81 (MQ=255)                                                                    gTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGcc        >  2:757293/1‑81 (MQ=255)                                                                    gTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGcc        >  1:757297/1‑81 (MQ=255)                                                                    gTGGATCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGcc        <  1:757200/81‑1 (MQ=255)                                                                         tCCGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGatat   <  2:757201/81‑1 (MQ=255)                                                                           cGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATc  <  2:757205/80‑1 (MQ=255)                                                                           cGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATc  <  1:757203/80‑1 (MQ=255)                                                                           cGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATc  <  2:757204/80‑1 (MQ=255)                                                                           cGGCCGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATc  <  2:757202/80‑1 (MQ=255)                                                                               |                                                                            CGCGTGGCAGGAAATTGAACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACAACAGACGCCGTGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATC  >  NC_000913/3887691‑3887844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑