Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,896,626	A→C	intergenic (‑17/‑148)	adeP ← / → yieH	adenine:H(+) symporter/6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,896,626	0	A	C	100.0%	138.2 / NA	38	intergenic (‑17/‑148)	adeP/yieH	adenine:H(+) symporter/6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/24);  total (14/24)

TTTAAACACGCGTTCCAGCATCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACAAGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTTGTGAGAGACTTTTTTATTGGATT  >  NC_000913/3896550‑3896701                                                                             |                                                                            tttAAACACGCGTTCCAGCATCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACAcggag                                                                         >  2:759424/1‑81 (MQ=255)   tAAACACGCGTTCCAGCATCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACAcggagg                                                                        >  2:759427/1‑80 (MQ=255)   tAAACACGCGTTCCAGCATCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACAcggagg                                                                        <  1:759369/80‑1 (MQ=255)        acGCGTTCCAGCATCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAAt                                                                  <  1:759370/81‑1 (MQ=255)        acGCGTTCCAGCATCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAAt                                                                  <  1:759372/81‑1 (MQ=255)        acGCGTTCCAGCATCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAAt                                                                  <  2:759371/81‑1 (MQ=255)            gTTCCAGCATCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGt                                                              <  2:759374/81‑1 (MQ=255)            gTTCCAGCATCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGt                                                              <  2:759375/81‑1 (MQ=255)            gTTCCAGCATCCCCTGACCAGAAGTCTGGGTAGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGt                                                              <  2:759373/81‑1 (MQ=255)             ttCCAGCATCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTc                                                             <  1:759376/81‑1 (MQ=255)                  gCATCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTAt                                                        <  1:759377/81‑1 (MQ=255)                    aTCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCg                                                      >  1:759416/1‑81 (MQ=255)                    aTCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCg                                                      <  1:759378/81‑1 (MQ=255)                           gACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATAcc                                                >  1:759415/1‑80 (MQ=255)                           gACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATAcc                                                >  2:759421/1‑80 (MQ=255)                            aCCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCa                                               <  1:759379/80‑1 (MQ=255)                               aGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAAt                                           <  2:759380/81‑1 (MQ=255)                                  aGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGc                                         >  2:759422/1‑80 (MQ=255)                                    tCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGAc                                      <  1:759381/81‑1 (MQ=255)                                     cTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGAc                                      >  2:759432/1‑80 (MQ=255)                                         gtggtATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAg                                 <  1:759382/81‑1 (MQ=255)                                             tATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCGGTCGCTATCGTATACCAAAATGCGACAATAGgcgc                             >  2:759441/1‑81 (MQ=255)                                             tATGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGgcgc                             <  1:759383/81‑1 (MQ=255)                                              aTGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGcgcg                            >  2:759429/1‑81 (MQ=255)                                               tGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGcgcg                            <  2:759385/80‑1 (MQ=255)                                               tGTTGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGcgcg                            <  2:759384/80‑1 (MQ=255)                                                 ttGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGttt                         <  1:759387/81‑1 (MQ=255)                                                 ttGATGACTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGtt                          <  1:759386/80‑1 (MQ=255)                                                   gatgaCTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTtgt                       >  1:759425/1‑81 (MQ=255)                                                   gatgaCTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTtgt                       >  2:759428/1‑81 (MQ=255)                                                   gatgaCTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTtg                        <  1:759388/80‑1 (MQ=255)                                                    atgaCTCATTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTtgtg                      <  2:759389/81‑1 (MQ=255)                                                           aTTATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTTGTGAGAGACt               >  2:759430/1‑81 (MQ=255)                                                             tATCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTTGTGAGAGACttt             >  2:759434/1‑81 (MQ=255)                                                               tCTATTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTTGTGAGAGACtttt            <  2:759390/80‑1 (MQ=255)                                                                  aTTTCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTTGTGAGAGACTTTTTTAt        >  1:759426/1‑81 (MQ=255)                                                                   tttCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTTGTGAGAGACtttttt          <  2:759391/78‑1 (MQ=255)                                                                   tttCCGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTTGTGAGAGACTTTTTTa         <  1:759392/79‑1 (MQ=255)                                                                      ccGAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTTGTGAGAGACTTTTTTATTgg     <  2:759393/80‑1 (MQ=255)                                                                        gAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTTGTGAGAGACTTTTTTATTGGAtt  >  2:759431/1‑81 (MQ=255)                                                                        gAACACGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTTGTGAGAGACTTTTTTATTGGAtt  >  2:759433/1‑81 (MQ=255)                                                                             |                                                                            TTTAAACACGCGTTCCAGCATCCCCTGACCAGAAGTCTGGGTGGTATGTTGATGACTCATTATCTATTTCCGAACAAGGAGGGAAAATCCGTCGCTATCGTATACCAAAATGCGACAATAGGCGCGTTTGTGAGAGACTTTTTTATTGGATT  >  NC_000913/3896550‑3896701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑