Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,899,560	C→T	D340N (GAT→AAT)	yieL ←	putative hydrolase YieL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,899,560	0	C	T	100.0%	120.7 / NA	33	D340N (GAT→AAT)	yieL	putative hydrolase YieL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/11);  total (22/11)

TTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGA  >  NC_000913/3899483‑3899634                                                                              |                                                                           ttCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACAtttt                                                                          <  1:760035/80‑1 (MQ=255)   ccTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTAt                                                                       >  1:760062/1‑81 (MQ=255)          aTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCca                                                                 >  1:760068/1‑80 (MQ=255)           tCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCcaca                                                               <  1:760036/81‑1 (MQ=255)           tCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCcaca                                                               <  1:760037/81‑1 (MQ=255)             aaaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAAc                                                             >  1:760080/1‑81 (MQ=255)                   aaTTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAg                                                        <  1:760038/80‑1 (MQ=255)                      ttttttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTaa                                                     >  1:760140/1‑80 (MQ=255)                        ttttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAAt                                                  >  2:760074/1‑81 (MQ=255)                          ttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTa                                                >  1:760093/1‑81 (MQ=255)                          ttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTa                                                >  2:760090/1‑81 (MQ=255)                              gCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACg                                              >  1:760065/1‑79 (MQ=255)                                  aaGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTg                                        >  1:760070/1‑81 (MQ=255)                                    gCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCt                                      >  1:760069/1‑81 (MQ=255)                                     cTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTg                                     <  1:760039/81‑1 (MQ=255)                                       cAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGt                                   >  1:760078/1‑81 (MQ=255)                                        aGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTgg                                    <  2:760040/79‑1 (MQ=255)                                           tttCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGa                                >  2:760071/1‑80 (MQ=255)                                            ttCAGCCCGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGa                                >  1:760075/1‑79 (MQ=255)                                              cAGCCCGGCGATATCCTTGCCGGATACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGc                            >  1:760097/1‑81 (MQ=255)                                                 cccGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGc                         >  1:760076/1‑81 (MQ=255)                                                  ccGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGcc                        >  1:760098/1‑81 (MQ=255)                                                   cGGCGATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCg                       >  1:760072/1‑81 (MQ=255)                                                       gATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATc                   >  1:760088/1‑81 (MQ=255)                                                       gATATCCTTGCCGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATc                   <  1:760041/81‑1 (MQ=255)                                                                 ccGGTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCgg         <  1:760042/81‑1 (MQ=255)                                                                    gTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGcc      >  2:760091/1‑81 (MQ=255)                                                                    gTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGcc      >  2:760086/1‑81 (MQ=255)                                                                    gTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGcc      <  2:760044/81‑1 (MQ=255)                                                                    gTTACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGcc      <  1:760043/81‑1 (MQ=255)                                                                      tACGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcgc    >  1:760073/1‑81 (MQ=255)                                                                       aCGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcgc    <  1:760045/80‑1 (MQ=255)                                                                        cGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcgcg   <  1:760046/80‑1 (MQ=255)                                                                        cGACATTTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGa  >  1:760047/1‑81 (MQ=255)                                                                              |                                                                           TTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGA  >  NC_000913/3899483‑3899634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑