Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,908,040	G→A	D109D (GAC→GAT)	pstB ←	phosphate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,908,040	0	G	A	100.0%	149.4 / NA	41	D109D (GAC→GAT)	pstB	phosphate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/23);  total (18/23)

GGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCT  >  NC_000913/3907962‑3908116                                                                               |                                                                             ggTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATc                                                                            >  1:762004/1‑81 (MQ=255)  gTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATc                                                                            <  1:761949/80‑1 (MQ=255)     aaTGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAg                                                                        <  1:761950/81‑1 (MQ=255)     aaTGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAg                                                                        <  2:761951/81‑1 (MQ=255)       tGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGAt                                                                      >  1:762011/1‑81 (MQ=255)         cccACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATgg                                                                    <  1:761952/81‑1 (MQ=255)          ccACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGa                                                                   <  1:761953/81‑1 (MQ=255)           cACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGAc                                                                  <  1:761955/81‑1 (MQ=255)           cACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGAc                                                                  <  2:761954/81‑1 (MQ=255)            aCTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACa                                                                 >  2:762013/1‑81 (MQ=255)                cACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCg                                                              <  1:761956/80‑1 (MQ=255)                cACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCg                                                              >  1:762032/1‑80 (MQ=255)                 aCGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCgg                                                             >  2:762015/1‑80 (MQ=255)                   gcgcTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGaaa                                                          >  2:762018/1‑81 (MQ=255)                     gcTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACg                                                        <  2:761957/81‑1 (MQ=255)                        cGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCg                                                     >  2:762000/1‑81 (MQ=255)                          tCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTc                                                   <  2:761960/81‑1 (MQ=255)                          tCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTc                                                   <  2:761959/81‑1 (MQ=255)                          tCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTc                                                   <  2:761958/81‑1 (MQ=255)                           ccATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCg                                                  >  2:762012/1‑81 (MQ=255)                                tCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGttt                                              <  1:761961/80‑1 (MQ=255)                                tCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTc                                             <  1:761962/81‑1 (MQ=255)                                  ggCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCt                                            <  1:761963/80‑1 (MQ=255)                                   gCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTg                                           >  1:762022/1‑80 (MQ=255)                                      cGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAac                                       <  1:761964/81‑1 (MQ=255)                                       gggAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAaca                                      >  2:762014/1‑81 (MQ=255)                                         gagaGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAacac                                     >  2:762029/1‑80 (MQ=255)                                         gagaGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACAcc                                    <  2:761965/81‑1 (MQ=255)                                          agagCTTCTCAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCa                                   >  2:762008/1‑81 (MQ=255)                                                 ctcAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGccc                              <  1:761966/79‑1 (MQ=255)                                                   cAAACAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACtt                          >  2:762028/1‑81 (MQ=255)                                                    aaaCAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACt                           <  1:761967/79‑1 (MQ=255)                                                       cAGACGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgc                      <  2:761968/81‑1 (MQ=255)                                                          aCGAACGCCAAAAGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgcacg                   >  1:762020/1‑81 (MQ=255)                                                                  cAAAAGCGATGTTATCGTAGATGGACATCGGGAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTg           <  2:761969/81‑1 (MQ=255)                                                                   aaaaGCGATGTTATCGTAGATGGACATCGGAGACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGc          <  2:761970/81‑1 (MQ=255)                                                                     aaGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGa        >  2:762021/1‑81 (MQ=255)                                                                     aaGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGa        >  2:762044/1‑81 (MQ=255)                                                                      aGCGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGa        >  2:762016/1‑80 (MQ=255)                                                                        cGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGatat     <  1:761971/81‑1 (MQ=255)                                                                        cGATGTTATCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGatat     >  2:762038/1‑81 (MQ=255)                                                                            gttatCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCt  >  2:762054/1‑80 (MQ=255)                                                                               |                                                                             GGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCT  >  NC_000913/3907962‑3908116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑