Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,025,344	C→T	N119N (AAC→AAT)	ubiD →	3‑octaprenyl‑4‑hydroxybenzoate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,025,344	0	C	T	100.0%	116.4 / NA	32	N119N (AAC→AAT)	ubiD	3‑octaprenyl‑4‑hydroxybenzoate decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/15);  total (17/15)

GTTTCTGAAAGAGCCGGAGCCGCCAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAACATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGATGACGTCGATCTCAATCG  >  NC_000913/4025266‑4025418                                                                               |                                                                           gTTTCTGAAAGAGCCGGAGCCGCCAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAatat                                                                          <  2:792586/81‑1 (MQ=255) gTTTCTGAAAGAGCCGGAGCCGCCAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAatat                                                                          <  2:792585/81‑1 (MQ=255)   ttCTGAAAGAGCCGGAGCCGCCAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGc                                                                        >  1:792624/1‑81 (MQ=255)     cTGAAAGAGCCGGAGCCGCCAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGcc                                                                       >  1:792631/1‑80 (MQ=255)          agagCCGGAGCCGCCAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACaaa                                                                 <  1:792587/81‑1 (MQ=255)             gccggagccgCCAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCg                                                              <  2:792588/81‑1 (MQ=255)               cggagccgCCAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGc                                                            >  2:792638/1‑81 (MQ=255)                 gagccgCCAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTg                                                          <  1:792589/81‑1 (MQ=255)                  agccgCCAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGc                                                         >  2:792642/1‑81 (MQ=255)                      gccAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCgtgg                                                     >  1:792626/1‑81 (MQ=255)                         aaaaGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTgc                                                  <  2:792590/81‑1 (MQ=255)                            aGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGcc                                               >  1:792630/1‑81 (MQ=255)                              gTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCt                                             >  1:792647/1‑81 (MQ=255)                              gTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCt                                             <  2:792591/81‑1 (MQ=255)                                 tCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGc                                           <  1:792592/80‑1 (MQ=255)                                   cgcgACCTGTTTGATAAACTGACGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCcaa                                        >  2:792645/1‑81 (MQ=255)                                      gACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCcaaca                                      >  2:792649/1‑80 (MQ=255)                                            tttGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATc                               >  1:792656/1‑81 (MQ=255)                                             ttGATAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCg                              >  1:792636/1‑81 (MQ=255)                                                aTAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGtct                           >  1:792641/1‑81 (MQ=255)                                                 tAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGtctc                          >  2:792646/1‑81 (MQ=255)                                                 tAAACTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGtctc                          <  2:792593/81‑1 (MQ=255)                                                   aaCTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTctct                         <  1:792594/80‑1 (MQ=255)                                                     cTGCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGc                      <  2:792595/81‑1 (MQ=255)                                                       gCCGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGa                    >  1:792644/1‑81 (MQ=255)                                                        ccGCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGAt                   >  1:792648/1‑81 (MQ=255)                                                         cGCAGTTTAAGTAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGAt                   >  2:792661/1‑80 (MQ=255)                                                          gCAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGATGa                 <  2:792596/81‑1 (MQ=255)                                                           cAGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGATGAc                <  1:792597/81‑1 (MQ=255)                                                            aGTTTAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGATGACg               <  2:792598/81‑1 (MQ=255)                                                                tAAGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGATGACGTCg            <  2:792599/80‑1 (MQ=255)                                                                  aGCAAGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGATGACGTCGAtc         <  2:792600/81‑1 (MQ=255)                                                                     aaGTATTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGATGACGTCGATCTCa      <  2:792601/81‑1 (MQ=255)                                                                         aTTGAATATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGATGACGTCGATCTCAATCg  >  2:792665/1‑81 (MQ=255)                                                                               |                                                                           GTTTCTGAAAGAGCCGGAGCCGCCAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAACATGCCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGATGACGTCGATCTCAATCG  >  NC_000913/4025266‑4025418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑