Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,027,665	C→T	A370T (GCG→ACG)	fadA ←	3‑ketoacyl‑CoA thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,027,665	0	C	T	100.0%	133.1 / NA	37	A370T (GCG→ACG)	fadA	3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/18);  total (19/18)

GCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGC  >  NC_000913/4027591‑4027741                                                                           |                                                                             gccgcATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGAcc                                                                        <  2:793158/81‑1 (MQ=255)    gcATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCaaa                                                                     >  2:793268/1‑81 (MQ=255)    gcATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCaaa                                                                     >  2:793215/1‑81 (MQ=255)    gcATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCaaa                                                                     >  2:793231/1‑81 (MQ=255)        ccGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTg                                                                  >  1:793219/1‑80 (MQ=255)             aaGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTc                                                            >  2:793222/1‑81 (MQ=255)             aaGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTc                                                            >  1:793241/1‑81 (MQ=255)             aaGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTc                                                            >  2:793234/1‑81 (MQ=255)               gTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCtt                                                          <  2:793159/81‑1 (MQ=255)                tGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCttt                                                         <  2:793160/81‑1 (MQ=255)                  gTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTg                                                        <  1:793161/80‑1 (MQ=255)                  gTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGc                                                       <  2:793162/81‑1 (MQ=255)                        cccGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCa                                                 >  2:793230/1‑81 (MQ=255)                          cGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATc                                               <  2:793163/81‑1 (MQ=255)                          cGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATc                                               <  2:793164/81‑1 (MQ=255)                               aaaCACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGa                                            <  2:793165/79‑1 (MQ=255)                               aaaCACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGAtt                                          >  2:793243/1‑81 (MQ=255)                                   acCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTcag                                       >  1:793246/1‑80 (MQ=255)                                    ccGTCGCAATACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTcagca                                     <  2:793166/81‑1 (MQ=255)                                           aaTACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCgtggt                              <  2:793167/81‑1 (MQ=255)                                           aaTACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCgtggt                              >  1:793244/1‑81 (MQ=255)                                            aTACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCgtggtg                             <  2:793168/81‑1 (MQ=255)                                             tACCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGc                            >  1:793235/1‑81 (MQ=255)                                              aCCCTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCt                           >  2:793225/1‑81 (MQ=255)                                                 cTGACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGAt                        <  2:793169/81‑1 (MQ=255)                                                   gACCCAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATAc                      >  1:793240/1‑81 (MQ=255)                                                       cAGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATAcgcg                   >  2:793239/1‑80 (MQ=255)                                                        aGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCa                 >  2:793250/1‑81 (MQ=255)                                                        aGACCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCa                 >  1:793232/1‑81 (MQ=255)                                                          aCCGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCAcc               >  2:793170/1‑81 (MQ=255)                                                           ccGATACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCg              >  2:793245/1‑81 (MQ=255)                                                              aTACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGaa           <  1:793171/81‑1 (MQ=255)                                                               tACACATCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGaac          <  1:793172/81‑1 (MQ=255)                                                                 cacaTCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGaaca         <  2:793174/80‑1 (MQ=255)                                                                 cacaTCGTCGTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGaaca         <  2:793173/80‑1 (MQ=255)                                                                     tcgtcgTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCa    <  2:793176/81‑1 (MQ=255)                                                                     tcgtcgTCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCa    <  2:793175/81‑1 (MQ=255)                                                                       gtcgtcAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAg   <  2:793177/80‑1 (MQ=255)                                                                       gtcgtcAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGc  <  2:793178/81‑1 (MQ=255)                                                                           |                                                                             GCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGC  >  NC_000913/4027591‑4027741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑