Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	399,509	T→C	intergenic (‑176/‑84)	yaiY ← / → yaiZ	inner membrane protein/DUF2754 domain‑containing protein YaiZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	399,509	0	T	C	100.0%	82.0 / NA	24	intergenic (‑176/‑84)	yaiY/yaiZ	inner membrane protein/DUF2754 domain‑containing protein YaiZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/11);  total (13/11)

GATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTAA  >  NC_000913/399438‑399582                                                                        |                                                                          gATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAAtt                                                                   <  1:88134/80‑1 (MQ=255)    gTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACg                                                                >  2:88177/1‑80 (MQ=255)           atatTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAAtgtt                                                        >  2:88165/1‑81 (MQ=255)            tatTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTATGTTAAATTTACTAACAATTACGAAAAtgttg                                                       >  2:88162/1‑81 (MQ=255)              ttCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTGAATTTACTAACAATTACGAAAAtgttgt                                                      <  1:88135/80‑1 (MQ=255)                  cGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTaca                                                 >  2:88166/1‑81 (MQ=255)                  cGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTaca                                                 >  2:88172/1‑81 (MQ=255)                     aGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTAcacca                                              >  2:88158/1‑81 (MQ=255)                      gCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTAcaccac                                             <  2:88136/81‑1 (MQ=255)                      gCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTAcaccac                                             <  2:88137/81‑1 (MQ=255)                              cTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGgtt                                     >  2:88170/1‑81 (MQ=255)                                 cGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGgttg                                    <  1:88138/79‑1 (MQ=255)                                  gTTGTGAGTCA‑AAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGttgttg                                 >  2:88169/1‑80 (MQ=255)                                   ttGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGc                                <  2:88139/81‑1 (MQ=255)                                             caAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGc                       >  2:88168/1‑80 (MQ=255)                                              aaaaTGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGc                       >  2:88163/1‑79 (MQ=255)                                              aaaaTGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCaa                     <  1:88140/81‑1 (MQ=255)                                               aaaTGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAg                    >  1:88161/1‑81 (MQ=255)                                                    gCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGAtgt               <  2:88141/81‑1 (MQ=255)                                                    gCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGAtgt               >  1:88175/1‑81 (MQ=255)                                                      aaCCTTAAGTTAAATTTACTAACACTTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGAtgttg             >  2:88167/1‑81 (MQ=255)                                                            aaGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGtttt         <  1:88142/79‑1 (MQ=255)                                                                  aaTTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTaa  <  1:88143/80‑1 (MQ=255)                                                                  aaTTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTaa  <  1:88144/80‑1 (MQ=255)                                                                        |                                                                          GATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTAA  >  NC_000913/399438‑399582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑