Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,054,735	G→A	H182Y (CAT→TAT)	glnG ←	DNA‑binding transcriptional dual regulator NtrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,054,735	0	G	A	100.0%	142.1 / NA	39	H182Y (CAT→TAT)	glnG	DNA‑binding transcriptional dual regulator NtrC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/19);  total (20/19)

TGATTCGATCAAATCTTTTGGGATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATGCAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCC  >  NC_000913/4054658‑4054812                                                                              |                                                                              tGATTCGATCAAATCTTTTGGGATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAg                                                                            >  1:799949/1‑81 (MQ=255)   aTTCGATCAAATCTTTTGGGATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAg                                                                            <  1:799881/79‑1 (MQ=255)    ttCGATCAAATCTTTTGGGATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAggg                                                                          >  2:799947/1‑80 (MQ=255)       gATCAAATCTTTTGGGATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCAt                                                                       <  1:799883/80‑1 (MQ=255)       gATCAAATCTTTTGGGATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCAt                                                                       >  2:799976/1‑80 (MQ=255)       gATCAAATCTTTTGGGATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATg                                                                      <  2:799884/81‑1 (MQ=255)       gATCAAATCTTTTGGGATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATg                                                                      <  2:799882/81‑1 (MQ=255)            aaTCTTTTGGGATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGa                                                                 <  2:799885/81‑1 (MQ=255)             aTCTTTTGGGATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGAc                                                                <  1:799886/81‑1 (MQ=255)                   tGGGATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGtt                                                           <  2:799887/80‑1 (MQ=255)                   tGGGATAGCTGCCATATTCAGCGCGATAAAAGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTc                                                          >  1:799959/1‑81 (MQ=255)                     ggATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCt                                                         <  1:799888/80‑1 (MQ=255)                      gATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCtt                                                        >  2:799950/1‑80 (MQ=255)                       aTAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTa                                                      >  1:799952/1‑81 (MQ=255)                         aGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTAcc                                                    >  1:799954/1‑81 (MQ=255)                             gCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTg                                                >  2:799938/1‑81 (MQ=255)                              ccATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGc                                               <  1:799889/81‑1 (MQ=255)                                atatTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCg                                             >  1:799962/1‑81 (MQ=255)                                atatTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCg                                             >  1:799972/1‑81 (MQ=255)                                atatTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCg                                             >  1:799946/1‑81 (MQ=255)                                 tatTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCg                                             >  1:799944/1‑80 (MQ=255)                                   ttCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGa                                           <  1:799890/80‑1 (MQ=255)                                           gATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGt                                   >  2:799948/1‑80 (MQ=255)                                            aTAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGtt                                  >  1:799957/1‑80 (MQ=255)                                            aTAAACGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTa                                 <  2:799891/81‑1 (MQ=255)                                               aaCGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAAt                               >  2:799968/1‑80 (MQ=255)                                               aaCGACGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAAt                               >  2:799973/1‑80 (MQ=255)                                                 cGGCGCTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAg                            >  2:799974/1‑81 (MQ=255)                                                      cTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGc                       <  1:799892/81‑1 (MQ=255)                                                      cTTTGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGc                       >  2:799961/1‑81 (MQ=255)                                                         tGGCGCGCGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTa                     <  2:799893/80‑1 (MQ=255)                                                           gcgcgcGGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAAt                   <  1:799894/80‑1 (MQ=255)                                                             gcgcgGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATag                 >  1:799966/1‑80 (MQ=255)                                                               gcgGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATagaag              <  2:799895/81‑1 (MQ=255)                                                                cgGACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATagaaga             <  1:799896/81‑1 (MQ=255)                                                                  gACTGTGGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAAc           >  1:799953/1‑81 (MQ=255)                                                                       tgGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAAcgcg        <  1:799898/79‑1 (MQ=255)                                                                       tgGCGATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGa       <  1:799897/80‑1 (MQ=255)                                                                           gATACAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGcc  <  2:799899/81‑1 (MQ=255)                                                                              |                                                                              TGATTCGATCAAATCTTTTGGGATAGCTGCCATATTCAGCGCGATAAACGGCGCTTTGGCGCGCGGACTGTGGCGATGCAGGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCC  >  NC_000913/4054658‑4054812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑