| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,063,091 | T→A | I282N (ATC→AAC) | yihN → | putative transporter YihN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,063,091 | 0 | T | A | 97.1% | 118.5 / ‑3.4 | 34 | I282N (ATC→AAC) | yihN | putative transporter YihN |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (15/18); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
GGGGGCTTACGGCATCATTAACCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGATCCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTGCGCTCGTACTGTTGATTATGCTG > NC_000913/4063014‑4063167 | gggggCTTACGGCATCATTAACCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCt > 2:802027/1‑81 (MQ=255) ggggCTTACGGCATCATTAACCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCt < 1:801996/80‑1 (MQ=255) ggggCTTACGGCATCATTAACCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTg < 2:801995/81‑1 (MQ=255) aCGGCATCATTAACCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATc < 2:801997/79‑1 (MQ=255) cGGCATCATTAACCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGcc < 1:801999/81‑1 (MQ=255) cGGCATCATTAACCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGcc < 1:802000/81‑1 (MQ=255) cGGCATCATTAACCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGc < 2:801998/80‑1 (MQ=255) gCATCATTAACCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGcc > 1:802037/1‑79 (MQ=255) tcatTAACCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTa < 1:802001/81‑1 (MQ=255) aCCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATc > 2:802031/1‑81 (MQ=255) aCCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATc < 1:802002/81‑1 (MQ=255) ccAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATc > 2:802050/1‑80 (MQ=255) aaTACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTAt < 2:802003/81‑1 (MQ=255) aTACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATg < 1:802004/81‑1 (MQ=255) tCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTacac < 2:802005/81‑1 (MQ=255) tGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCt > 2:802038/1‑81 (MQ=255) aaaaTGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCtt > 2:802043/1‑80 (MQ=255) aaaaTGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCtt < 1:802006/80‑1 (MQ=255) tGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCa > 2:802036/1‑81 (MQ=255) aTTGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCAt > 1:802042/1‑80 (MQ=255) ttGGTGGACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTa < 2:802007/81‑1 (MQ=255) tggACCGATTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTAc > 1:802045/1‑81 (MQ=255) gATTGGTGGCATGATTTCAGATAAGATCCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTgc > 2:802055/1‑81 (MQ=255) aTTGGTGGCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTgcg > 1:802054/1‑81 (MQ=255) gtggCATGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTGCGCTCg > 2:802039/1‑81 (MQ=255) tGATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTGCGCTCGTACTGt < 2:802008/81‑1 (MQ=255) gATTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTGCGCTCGTACTGtt > 1:802056/1‑81 (MQ=255) aTTTCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTGCGCTCGTACTGtt > 2:802041/1‑80 (MQ=255) tCAGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTGCGCTCGTACTGTTGAt < 1:802009/80‑1 (MQ=255) aGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTGCGCTCGTACTGTTGATTAt < 1:802010/81‑1 (MQ=255) aGATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTGCGCTCGTACTGTTGATTAt > 2:802046/1‑81 (MQ=255) gATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTGCGCTCGTACTGTTGATTAtg < 1:802011/81‑1 (MQ=255) gATAAGAACCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTGCGCTCGTACTGTTGATTAtg > 2:802070/1‑81 (MQ=255) aGAACCTGAAATCGCCAAGTAAATATCTATGCGACACCTTTATCATTAGTACCGCTGCGCTCGTACTGTTGATTAtgctg < 2:802012/80‑1 (MQ=255) | GGGGGCTTACGGCATCATTAACCAATACTGTCTGAAAATGATTGGTGGACCGATTGGTGGCATGATTTCAGATAAGATCCTGAAATCGCCAAGTAAATATCTATGCTACACCTTTATCATTAGTACCGCTGCGCTCGTACTGTTGATTATGCTG > NC_000913/4063014‑4063167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |