Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,109,886	T→C	intergenic (+63/‑44)	sbp → / → cdh	sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp/CDP‑diacylglycerol diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,109,886	0	T	C	100.0%	109.3 / NA	31	intergenic (+63/‑44)	sbp/cdh	sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp/CDP‑diacylglycerol diphosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/17);  total (14/17)

ATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGCGGGTCTTCTTTTTTTGGTGATGA  >  NC_000913/4109809‑4109963                                                                              |                                                                              atcagCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGActtt                                                                            <  2:813372/81‑1 (MQ=255) atcagCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGActtt                                                                            <  1:813371/81‑1 (MQ=255)    agCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCg                                                                         <  1:813373/81‑1 (MQ=255)       aaaCGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTa                                                                      <  2:813374/81‑1 (MQ=255)          cGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTAccc                                                                   >  1:813417/1‑81 (MQ=255)          cGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTAccc                                                                   >  1:813426/1‑81 (MQ=255)            cTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCtt                                                                 >  1:813422/1‑81 (MQ=255)              caTTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGTCCGGGTTTTTTTTTTGTCACGACTTTGCGTTACCCTTGc                                                               >  2:813410/2‑81 (MQ=255)                tttCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCAt                                                             <  2:813375/81‑1 (MQ=255)                  tCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCAtc                                                            <  2:813376/80‑1 (MQ=255)                   ccccAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCAtct                                                           <  1:813377/80‑1 (MQ=255)                      cAGGATAATCATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCttt                                                       >  1:813413/1‑81 (MQ=255)                          aTAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAgg                                                   >  1:813416/1‑81 (MQ=255)                          aTAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAgg                                                   <  1:813378/81‑1 (MQ=255)                             aTTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTa                                                 <  1:813380/80‑1 (MQ=255)                             aTTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTAc                                                <  2:813379/81‑1 (MQ=255)                               tATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAg                                              >  2:813418/1‑81 (MQ=255)                               tATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAg                                              >  1:813420/1‑81 (MQ=255)                                aTCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAg                                              <  2:813381/80‑1 (MQ=255)                                   aaaCCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGa                                           <  2:813382/80‑1 (MQ=255)                                      cccGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGaaaaa                                       <  1:813383/81‑1 (MQ=255)                                      cccGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGaaaa                                        >  2:813415/1‑80 (MQ=255)                                        cGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGaaaaaaa                                     <  1:813384/81‑1 (MQ=255)                                             gtTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATg                                 >  1:813414/1‑80 (MQ=255)                                             gtTTCTCGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATg                                 >  1:813412/1‑80 (MQ=255)                                                 ctcGCGACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGaaaa                             <  2:813385/80‑1 (MQ=255)                                                      gACCGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGCgg                       >  2:813430/1‑81 (MQ=255)                                                         cGGGTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGCGGGtc                    <  2:813386/81‑1 (MQ=255)                                                           ggTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGCGGGtctt                  <  1:813388/81‑1 (MQ=255)                                                           ggTTTTTTATTTGTCACGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGCGGGtctt                  <  1:813387/81‑1 (MQ=255)                                                                   aTTTGTCACGACTTCGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGCGGGTCTTCtttttt           <  2:813389/80‑1 (MQ=255)                                                                          aCGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGCGGGTCTTCTTTTTTTGGtgat    >  1:813427/1‑80 (MQ=255)                                                                           cGACTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGCGGGTCTTCTTTTTTTGGtgatga  >  1:813423/1‑81 (MQ=255)                                                                              |                                                                              ATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGCGGGTCTTCTTTTTTTGGTGATGA  >  NC_000913/4109809‑4109963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑