Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,156,999 A→G L50L (TTA→TTG)  argH → argininosuccinate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,156,9990AG97.8% 161.3 / ‑4.1 46L50L (TTA→TTG) argHargininosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (22/23);  minor base T (0/1);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

TTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAAT  >  NC_000913/4156922‑4157076
                                                                             |                                                                             
tttGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGAcc                                                                            >  2:825781/1‑81 (MQ=255)
   gATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCg                                                                           >  2:825802/1‑79 (MQ=255)
    aTTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCg                                                                           >  1:825775/1‑78 (MQ=255)
         cGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAagag                                                                   <  2:825725/81‑1 (MQ=255)
            cTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAg                                                                >  2:825779/1‑81 (MQ=255)
            cTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAg                                                                >  2:825787/1‑81 (MQ=255)
            cTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAg                                                                >  2:825790/1‑81 (MQ=255)
                  gAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGCAGGCGTGTTGACCGCAGAAGAGCAGGCGCaa                                                          >  2:825797/1‑81 (MQ=255)
                   aGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAAc                                                         >  2:825755/1‑81 (MQ=255)
                   aGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAAc                                                         <  1:825726/81‑1 (MQ=255)
                    gCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACt                                                        <  1:825727/81‑1 (MQ=255)
                     cAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTg                                                       >  2:825810/1‑81 (MQ=255)
                         atatTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAag                                                   <  1:825729/81‑1 (MQ=255)
                         atatTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAag                                                   <  2:825728/81‑1 (MQ=255)
                          tatTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGaa                                                    >  1:825805/1‑79 (MQ=255)
                          tatTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAaga                                                  <  2:825730/81‑1 (MQ=255)
                            ttgttgGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAagag                                                 <  1:825731/80‑1 (MQ=255)
                                  gCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGa                                          <  2:825732/81‑1 (MQ=255)
                                        tgGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACgt                                      <  2:825733/79‑1 (MQ=255)
                                         ggCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGtt                                   >  2:825808/1‑81 (MQ=255)
                                          gCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGtt                                   <  2:825734/80‑1 (MQ=255)
                                          gCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGtt                                   <  2:825735/80‑1 (MQ=255)
                                           ccTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGc                                 >  2:825786/1‑81 (MQ=255)
                                            cTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCt                                <  2:825736/81‑1 (MQ=255)
                                                 ccAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTgg                              >  1:825795/1‑78 (MQ=255)
                                                 ccAAAGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTgg                              >  2:825782/1‑78 (MQ=255)
                                                   aaaGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGAt                         <  1:825737/81‑1 (MQ=255)
                                                   aaaGCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGAt                         >  2:825799/1‑81 (MQ=255)
                                                      gCCCTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGtt                      <  1:825738/81‑1 (MQ=255)
                                                       cccTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTc                     >  1:825796/1‑81 (MQ=255)
                                                        ccTGGTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTcg                    <  2:825739/81‑1 (MQ=255)
                                                            gTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGcc                <  2:825740/81‑1 (MQ=255)
                                                            gTCACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGcc                <  2:825741/81‑1 (MQ=255)
                                                              cACGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAg              >  1:825811/1‑81 (MQ=255)
                                                               aCGGTAGGCGTGTTTACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAg              <  2:825743/80‑1 (MQ=255)
                                                               aCGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAgg             <  2:825744/81‑1 (MQ=255)
                                                               aCGGTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAg              <  2:825742/80‑1 (MQ=255)
                                                                 ggTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGc            >  2:825794/1‑80 (MQ=255)
                                                                 ggTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGc            >  2:825803/1‑80 (MQ=255)
                                                                  gTAGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCca          <  1:825745/81‑1 (MQ=255)
                                                                    aGGCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCcac         >  1:825813/1‑80 (MQ=255)
                                                                      gCGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCacaac      <  2:825746/81‑1 (MQ=255)
                                                                       cGTGTTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCacaaca     <  2:825747/81‑1 (MQ=255)
                                                                         tgtTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACaaa   <  2:825748/81‑1 (MQ=255)
                                                                          gtTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAAt  >  1:825800/1‑81 (MQ=255)
                                                                          gtTGACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAAt  >  2:825823/1‑81 (MQ=255)
                                                                             |                                                                             
TTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAAT  >  NC_000913/4156922‑4157076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑