| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,164,740 | G→A | V368I (GTC→ATC) | btuB → | cobalamin/cobinamide outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,164,740 | 0 | G | A | 97.1% | 115.6 / ‑3.9 | 34 | V368I (GTC→ATC) | btuB | cobalamin/cobinamide outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (17/16); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
ACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAGTCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGGTCGTCATGGAACCTGGCAAAC > NC_000913/4164662‑4164816 | acgacgCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATc > 2:827643/1‑81 (MQ=255) acgCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGc < 1:827610/81‑1 (MQ=255) aGGTTATGTTGAGGATGGATATGATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCtt > 1:827655/1‑81 (MQ=255) tATGTTGAGGATGGATATGATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTg > 2:827648/1‑79 (MQ=255) aTGTTGAGGATGGATATGATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGaa < 1:827611/80‑1 (MQ=255) tGTTGAGGATGGATATGATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAgg < 2:827612/81‑1 (MQ=255) gAGGATGGATATGATCAACGTAATACAGGCGTCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGgcgc < 2:827613/80‑1 (MQ=255) aGGATGGATATGATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCgcag < 2:827615/81‑1 (MQ=255) aGGATGGATATGATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCgca < 1:827614/80‑1 (MQ=255) tGATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTg < 1:827617/80‑1 (MQ=255) tGATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTg < 2:827616/80‑1 (MQ=255) gATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGAc > 1:827631/1‑81 (MQ=255) tCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGa > 1:827653/1‑81 (MQ=255) tCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGa > 2:827665/1‑81 (MQ=255) cAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGa > 2:827662/1‑80 (MQ=255) aaCGTAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGAt > 2:827673/1‑80 (MQ=255) tAATACCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTc < 1:827618/81‑1 (MQ=255) aCCGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAg > 1:827649/1‑81 (MQ=255) ccGGCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATGACTCACAGt < 2:827619/81‑1 (MQ=255) ggCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGttt > 2:827694/1‑81 (MQ=255) ggCATCTATCTGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGttt < 2:827620/81‑1 (MQ=255) atctatctGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGgt > 2:827658/1‑81 (MQ=255) atctGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGgtcgt > 1:827672/1‑80 (MQ=255) tctGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGgtc < 2:827621/77‑1 (MQ=255) ctGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGGTCGTCAt > 2:827654/1‑81 (MQ=255) ctGACCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGGTCGTCAt > 2:827656/1‑81 (MQ=255) aCCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGGTCGTCAt < 2:827623/78‑1 (MQ=255) aCCGGGCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGGTCGTCATGGa < 2:827622/81‑1 (MQ=255) gggCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGGTCGTCATGGAAcc > 1:827660/1‑81 (MQ=255) ggCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGGTCGTCATGGAACCt < 2:827624/81‑1 (MQ=255) ggCTGCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGGTCGTCATGGAACCt < 2:827625/81‑1 (MQ=255) gCAACAAATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGGTCGTCATGGAACCTGGc > 2:827681/1‑80 (MQ=255) aacaaGTCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGGTCGTCATGGAACCTGGCaaa > 2:827663/1‑81 (MQ=255) acaaATCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGGTCGTCATGGAACCTGGCAAAc > 2:827666/1‑81 (MQ=255) | ACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATACCGGCATCTATCTGACCGGGCTGCAACAAGTCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGACGATAACTCACAGTTTGGTCGTCATGGAACCTGGCAAAC > NC_000913/4164662‑4164816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |