Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,177,057	G→A	G372S (GGT→AGT)	tufB →	translation elongation factor Tu 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,177,057	0	G	A	100.0%	40.6 / NA	14	G372S (GGT→AGT)	tufB	translation elongation factor Tu 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/3);  total (11/3)

CTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCC  >  NC_000913/4177030‑4177133                            |                                                                             cTGATCCACCCGATCGCGATGGACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTa                         <  2:830496/81‑1 (MQ=14)  tGATCCACCCGATCGCGATGGACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAg                        >  2:830532/1‑81 (MQ=17)   gATCCACCCGATCGCGATGGACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAg                        >  1:830540/1‑80 (MQ=17)    aTCCACCCGATCGCGATGGACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGc                       >  1:830566/1‑80 (MQ=21)    aTCCACCCGATCGCGATGGACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCa                      <  2:830497/81‑1 (MQ=25)         cccGATCGCGATGGACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGt                 <  1:830498/81‑1 (MQ=255)            gATCGCGATGGACTACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCt              >  2:830551/1‑81 (MQ=255)                 cgATGGACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGc          >  1:830549/1‑80 (MQ=255)                  gATGGACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCt         >  1:830550/1‑80 (MQ=255)                  gATGGACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCt         >  2:830544/1‑80 (MQ=255)                  gATGGACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCt         >  2:830560/1‑80 (MQ=255)                  gATGGACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCt         >  2:830577/1‑80 (MQ=255)                   aTGGACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTaa       >  2:830556/1‑81 (MQ=255)                     ggACGACAGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAAtt     >  2:830558/1‑81 (MQ=255)                       acgacaGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGc   >  1:830546/1‑81 (MQ=255)                         gacaGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGcc  >  2:830552/1‑80 (MQ=255)                            |                                                                             CTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCC  >  NC_000913/4177030‑4177133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑