Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,196,457	C→T	D118N (GAT→AAT)	rsd ←	regulator of sigma D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,196,457	0	C	T	100.0%	107.4 / NA	30	D118N (GAT→AAT)	rsd	regulator of sigma D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/16);  total (14/16)

CTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGT  >  NC_000913/4196385‑4196524                                                                         |                                                                    cTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGa                                                             >  2:835928/1‑81 (MQ=255)   tCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATg                                                           >  2:835929/1‑81 (MQ=255)    ccAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCg                                                              <  1:835884/77‑1 (MQ=255)    ccAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCg                                                              <  2:835885/77‑1 (MQ=255)    ccAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCg                                                              <  2:835883/77‑1 (MQ=255)    ccAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGAt                                                            <  2:835886/79‑1 (MQ=255)    ccAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATg                                                           <  2:835882/80‑1 (MQ=255)            aaGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTcc                                                  <  1:835887/81‑1 (MQ=255)                      ccAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGaa                                         <  2:835888/80‑1 (MQ=255)                      ccAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAAt                                        >  2:835944/1‑81 (MQ=255)                       cAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATc                                       <  2:835889/81‑1 (MQ=255)                         gcgcCTCGCTGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCgt                                     <  1:835890/81‑1 (MQ=255)                           gcCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCgtag                                   >  2:835934/1‑81 (MQ=255)                            ccTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCgtagt                                  <  1:835891/81‑1 (MQ=255)                             cTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCgtagt                                  >  2:835936/1‑80 (MQ=255)                                gCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATc                              >  1:835948/1‑81 (MQ=255)                                   gATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCa                            >  2:835939/1‑80 (MQ=255)                                   gATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCa                            >  2:835933/1‑80 (MQ=255)                                      gTCAGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAAt                        >  1:835924/1‑81 (MQ=255)                                         aGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCt                      <  2:835893/80‑1 (MQ=255)                                         aGATAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATAGCGGTTTCCAGACTAGAATCGTAGTAAACCATAATCTg                     <  2:835892/81‑1 (MQ=255)                                            tAAAACCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGtt                   >  2:835921/1‑80 (MQ=255)                                              aaaCCTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGa                 <  2:835894/80‑1 (MQ=255)                                                 ccTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTAt             >  1:835938/1‑81 (MQ=255)                                                 ccTGTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTAt             <  1:835895/81‑1 (MQ=255)                                                    gTTGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTgg          >  1:835946/1‑81 (MQ=255)                                                      tGAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCt        >  1:835932/1‑81 (MQ=255)                                                       gAAACTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCtt       >  1:835954/1‑81 (MQ=255)                                                        aaaCTCAAGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCtt       <  2:835896/80‑1 (MQ=255)                                                              aaGGTAATTATTATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGt  <  1:835897/79‑1 (MQ=255)                                                                         |                                                                    CTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGT  >  NC_000913/4196385‑4196524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑