Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	401,507	C→T	A41V (GCG→GTG)	iraP →	anti‑adaptor protein for sigma(S) stabilization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	401,507	0	C	T	100.0%	47.2 / NA	15	A41V (GCG→GTG)	iraP	anti‑adaptor protein for sigma(S) stabilization
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/6);  total (9/6)

AAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCC  >  NC_000913/401444‑401568                                                                |                                                              aaGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGc                                              <  1:88543/81‑1 (MQ=255)         tgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGttgat                                       <  1:88544/80‑1 (MQ=255)                 ggTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAgg                               >  2:88577/1‑80 (MQ=255)                   tAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAGGTag                            <  1:88545/81‑1 (MQ=255)                      aaGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAgg                         >  2:88587/1‑81 (MQ=255)                      aaGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAgg                         >  2:88590/1‑81 (MQ=255)                          tttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGgc                     >  1:88578/1‑81 (MQ=255)                             ggAGATTATCGTCACTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCt                  >  2:88575/1‑81 (MQ=255)                                     tCGTCACTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGCTGATTTATCAGGTAGAGGGGGCGCTGTACGAgg          >  1:88570/1‑81 (MQ=255)                                       gTCACTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTa        <  1:88546/81‑1 (MQ=255)                                       gTCACTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTa        <  2:88547/81‑1 (MQ=255)                                         cACTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTa        >  2:88579/1‑79 (MQ=255)                                          aCTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTaaa      <  2:88548/80‑1 (MQ=255)                                           cTGCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGc    >  1:88580/1‑81 (MQ=255)                                             gCAATGCTTCGCAATATGGTGCAAAATGACCAACAGCGGTTGATTGATCAGGCAGAGGGGGCGCTGTACGAGGTAAAGccc  >  1:88595/1‑81 (MQ=255)                                                                |                                                              AAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCC  >  NC_000913/401444‑401568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑