Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,265	G→A	intergenic (+56/‑213)	metA → / → aceB	homoserine O‑succinyltransferase/malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,265	0	G	A	100.0%	76.3 / NA	22	intergenic (+56/‑213)	metA/aceB	homoserine O‑succinyltransferase/malate synthase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/12);  total (10/12)

ACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATC  >  NC_000913/4215187‑4215338                                                                               |                                                                          acaTGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGCTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGt                                                                         <  2:840918/81‑1 (MQ=255)    tGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGcc                                                                      <  2:840919/81‑1 (MQ=255)      aaTCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCt                                                                     <  1:840920/80‑1 (MQ=255)        tCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCtttt                                                                  <  2:840921/81‑1 (MQ=255)         ccAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCttttt                                                                 >  2:840945/1‑81 (MQ=255)          cAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCttttt                                                                 <  1:840922/80‑1 (MQ=255)           aaCGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTa                                                               >  2:840950/1‑81 (MQ=255)               cTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTc                                                           <  1:840923/81‑1 (MQ=255)                     tAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTCCGAAAc                                                     >  1:840947/1‑81 (MQ=255)                      aaTCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTCCGAAAc                                                     <  1:840924/80‑1 (MQ=255)                                gATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTCCGAAACGTACCTcag                                            >  2:840946/1‑79 (MQ=255)                                 aTAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTCCGAAACGTACCTcagcag                                         >  1:840949/1‑81 (MQ=255)                                           gTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAAt                               >  2:840953/1‑81 (MQ=255)                                               aGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAAtttt                            <  1:840925/80‑1 (MQ=255)                                               aGCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTa                           >  1:840955/1‑81 (MQ=255)                                                gCGATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTAt                          <  1:840926/81‑1 (MQ=255)                                                  gATTCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTAtt                         >  1:840954/1‑80 (MQ=255)                                                   aTTCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCa                       <  1:840927/81‑1 (MQ=255)                                                     tCAGCACCTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCata                     >  2:840951/1‑81 (MQ=255)                                                       aGCACCTTACCTCAGGCACCTTCGAGTGCCATTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCatat                    <  2:840928/80‑1 (MQ=255)                                                            cTTACCTCAGGCACCTTCGAGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTAt              <  1:840929/81‑1 (MQ=255)                                                                          cTTCGAGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATc  >  2:840959/1‑79 (MQ=255)                                                                               |                                                                          ACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATC  >  NC_000913/4215187‑4215338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑