Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,254,080	C→T	R796H (CGT→CAT)	plsB ←	glycerol‑3‑phosphate 1‑O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,254,080	0	C	T	100.0%	162.1 / NA	44	R796H (CGT→CAT)	plsB	glycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/17);  total (27/17)

ATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCG  >  NC_000913/4254006‑4254157                                                                           |                                                                              aTAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGt                                                                         >  2:850420/1‑81 (MQ=255)  tAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTc                                                                        >  1:850418/1‑81 (MQ=255)       cGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAAACGTCAAATGCACGTCTGATg                                                                   >  2:850439/1‑81 (MQ=255)        gTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATg                                                                   >  1:850428/1‑80 (MQ=255)         ttttCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTa                                                                 <  1:850387/81‑1 (MQ=255)          tttCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTaa                                                                >  1:850427/1‑81 (MQ=255)            tCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAAt                                                               <  1:850388/80‑1 (MQ=255)              gccgcATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCa                                                             <  2:850390/80‑1 (MQ=255)              gccgcATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCa                                                             <  2:850389/80‑1 (MQ=255)                 gcATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTc                                                         <  2:850391/81‑1 (MQ=255)                     ccGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGc                                                      >  2:850447/1‑80 (MQ=255)                     ccGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGc                                                      >  2:850467/1‑80 (MQ=255)                      cGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGcc                                                     <  2:850393/80‑1 (MQ=255)                      cGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGcc                                                     <  2:850392/80‑1 (MQ=255)                          aaTTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCaa                                                >  2:850443/1‑81 (MQ=255)                           aTTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAAc                                               >  1:850433/1‑81 (MQ=255)                              ctctctGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGa                                            >  2:850432/1‑81 (MQ=255)                               tctctGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGa                                            >  2:850421/1‑80 (MQ=255)                                  ctGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATaaa                                        >  1:850445/1‑81 (MQ=255)                                     aTTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCt                                     >  1:850450/1‑81 (MQ=255)                                     aTTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCt                                     <  1:850394/81‑1 (MQ=255)                                         cccTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCAt                                 >  1:850452/1‑81 (MQ=255)                                           cTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCg                               >  1:850441/1‑81 (MQ=255)                                           cTTCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCg                               <  1:850395/81‑1 (MQ=255)                                            ttCGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGt                              >  1:850455/1‑81 (MQ=255)                                              cGCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGtt                             <  1:850396/80‑1 (MQ=255)                                               gCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTc                           >  2:850440/1‑81 (MQ=255)                                               gCCCTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTc                           <  1:850397/81‑1 (MQ=255)                                                cccTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCt                          >  1:850414/1‑81 (MQ=255)                                                cccTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCt                          >  2:850434/1‑81 (MQ=255)                                                cccTGCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCt                          <  1:850398/81‑1 (MQ=255)                                                    gCGTCGCACTCTCAATCGTCGAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCg                      >  2:850444/1‑81 (MQ=255)                                                    gCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCg                      >  2:850460/1‑81 (MQ=255)                                                    gCGTCGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCg                      >  2:850477/1‑81 (MQ=255)                                                        cGCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTc                  >  1:850449/1‑81 (MQ=255)                                                         gCACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCg                 >  1:850461/1‑81 (MQ=255)                                                          cACTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCgg                <  2:850399/81‑1 (MQ=255)                                                           aCTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCgg                >  2:850437/1‑80 (MQ=255)                                                           aCTCTCAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCgg                >  1:850446/1‑80 (MQ=255)                                                             tctcAATCGTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCAt             >  1:850466/1‑81 (MQ=255)                                                                     gTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCt      <  1:850400/80‑1 (MQ=255)                                                                     gTCAAATGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTa     <  2:850401/81‑1 (MQ=255)                                                                        aaaTGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCg  <  1:850403/81‑1 (MQ=255)                                                                        aaaTGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCg  <  1:850402/81‑1 (MQ=255)                                                                           |                                                                              ATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCG  >  NC_000913/4254006‑4254157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑