Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,260,908	A→G	N104D (AAC→GAC)	yjbM →	uncharacterized protein YjbM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,260,908	0	A	G	100.0%	45.5 / NA	14	N104D (AAC→GAC)	yjbM	uncharacterized protein YjbM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/7);  total (7/7)

GCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTAT  >  NC_000913/4260836‑4260974                                                                         |                                                                   gCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACGACAGCtt                                                             <  1:852135/80‑1 (MQ=255) gCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACGACAGCtt                                                             <  2:852134/80‑1 (MQ=255)      tataCATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACGACAGCTTAAAatt                                                       <  2:852136/81‑1 (MQ=255)                 aaGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACGACAGCTTAAAATTATTTGCTTCGc                                            >  1:852158/1‑81 (MQ=255)                  aGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACGACAGCTTAAAATTATTTGCTTCGCt                                           <  1:852137/81‑1 (MQ=255)                   gCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACGACAGCTTAAAATTATTTGCTTCGCTg                                          <  2:852138/81‑1 (MQ=255)                              tGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACGACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACt                               >  2:852167/1‑81 (MQ=255)                                 ttttttAACGTTTGTTAATGCCTGCCAGGAACATGATGACGACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACttt                             >  2:852170/1‑80 (MQ=255)                                        aCGTTTGTTAATGCCTGCCAGGAACATGATGACGACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCt                     <  2:852139/81‑1 (MQ=255)                                        aCGTTTGTTAATGCATGCCAGGAACATGATGACGACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCt                     <  1:852140/81‑1 (MQ=255)                                                  aTGCCTGCCAGGAACATGATGACGACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACg           >  2:852163/1‑81 (MQ=255)                                                         ccAGGAACATGATGACGACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGAtt     >  2:852161/1‑80 (MQ=255)                                                           aGGAACATGATGACGACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTAt  >  1:852169/1‑81 (MQ=255)                                                              aaCATGATGACGACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTAt  >  1:852168/1‑78 (MQ=255)                                                                         |                                                                   GCGAATATACATTTTCAAGCAATAGGTTCTGGTTTTTTAACGTTTGTTAATGCCTGCCAGGAACATGATGACAACAGCTTAAAATTATTTGCTTCGCTGTTAATTTCACTTTCATATTCTAGTGCCTACGCAGATTTAT  >  NC_000913/4260836‑4260974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑