Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,268,725	G→A	intergenic (+418/+84)	tyrB → / ← yjbS	tyrosine aminotransferase/uncharacterized protein YjbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,268,725	0	G	A	100.0%	27.6 / NA	9	intergenic (+418/+84)	tyrB/yjbS	tyrosine aminotransferase/uncharacterized protein YjbS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/2);  total (7/2)

TTTTTTCCGCTTTGTGTAAGTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTT  >  NC_000913/4268658‑4268802                                                                    |                                                                              ttttttCCGCTTTGTGTAAGTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATAAGAATAATCACCt                                                                  >  2:853931/1‑81 (MQ=255)     ttCCGCTTTGTGTAAGTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATAAGAATAATCACCTTTaa                                                              >  2:853933/1‑81 (MQ=255)             tgtgTAAGTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATAAGAATAATCACCTTTAACctcctc                                                       <  1:853919/80‑1 (MQ=255)                         aCCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATAAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCa                                           >  2:853935/1‑80 (MQ=255)                                   gATTACCAATATCAAATAAAAAGGTCCTGTAATAAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCaaa                                >  2:853938/1‑81 (MQ=255)                                               cAAATAAAAAGGTCCTGTAATAAGAATAATCACCTTTAACCTCCTCGGCTAAACAGCAAAGCGTGCAAATATACATAAAGt                    >  2:853934/1‑81 (MQ=255)                                               cAAATAAAAAGGTCCTGTAATAAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGt                    >  2:853937/1‑81 (MQ=255)                                                 aaTAAAAAGGTCCTGTAATAAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGtct                  <  2:853920/81‑1 (MQ=255)                                                                taataaGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGAt    <  1:853921/80‑1 (MQ=255)                                                                 aaTAAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGAttt  >  2:853943/1‑81 (MQ=255)                                                                    |                                                                              TTTTTTCCGCTTTGTGTAAGTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTT  >  NC_000913/4268658‑4268802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑