Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,272,989	T→A	I295F (ATC→TTC)	uvrA ←	excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,272,989	0	T	A	100.0%	112.9 / NA	31	I295F (ATC→TTC)	uvrA	excision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/17);  total (14/17)

GCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAAC  >  NC_000913/4272911‑4273065                                                                               |                                                                             gCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACa                                                                            >  1:854921/1‑81 (MQ=255)        gAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTc                                                                         <  2:854879/77‑1 (MQ=255)        gAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCg                                                                        <  2:854880/78‑1 (MQ=255)        gAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCg                                                                        <  2:854878/78‑1 (MQ=255)        gAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGAt                                                                      <  1:854881/80‑1 (MQ=255)        gAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGAt                                                                      >  2:854918/1‑80 (MQ=255)                 gAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGa                                                             >  2:854947/1‑80 (MQ=255)                       gcggcgATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTg                                                      >  2:854959/1‑81 (MQ=255)                        cggcgATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGc                                                     <  1:854882/81‑1 (MQ=255)                         ggcgATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCt                                                    <  2:854883/81‑1 (MQ=255)                         ggcgATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCt                                                    >  1:854932/1‑81 (MQ=255)                             aTCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTAc                                                <  1:854884/81‑1 (MQ=255)                             aTCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTAc                                                >  2:854942/1‑81 (MQ=255)                                ccAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGcc                                             <  1:854887/81‑1 (MQ=255)                                ccAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGcc                                             <  1:854886/81‑1 (MQ=255)                                ccAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGcc                                             <  1:854885/81‑1 (MQ=255)                                            cGCACCACCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCg                                   >  1:854941/1‑79 (MQ=255)                                              caccacCAGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAgg                               >  1:854952/1‑81 (MQ=255)                                                ccaccaGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTc                             <  2:854888/81‑1 (MQ=255)                                                 caccaGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCg                            <  1:854889/81‑1 (MQ=255)                                                  accaGCCAGCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCgg                           >  1:854944/1‑81 (MQ=255)                                                   ccagccagCGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCggg                          <  1:854890/81‑1 (MQ=255)                                                         agcGACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGcc                    >  1:854948/1‑81 (MQ=255)                                                            gACAGTTCCGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGccccc                 <  1:854891/81‑1 (MQ=255)                                                                   ccGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCggg           <  1:854892/80‑1 (MQ=255)                                                                    cGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGgtt         >  2:854937/1‑81 (MQ=255)                                                                    cGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGgtt         <  1:854893/81‑1 (MQ=255)                                                                    cGGATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGgtt         <  2:854894/81‑1 (MQ=255)                                                                      gATTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGgttgt       >  2:854935/1‑81 (MQ=255)                                                                       aTTCTGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGgttgt       >  2:854954/1‑80 (MQ=255)                                                                           tGGAACACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAAc  >  2:854938/1‑81 (MQ=255)                                                                               |                                                                             GCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAAC  >  NC_000913/4272911‑4273065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑