Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	427,572	G→A	D96N (GAC→AAC)	yajC →	Sec translocon accessory complex subunit YajC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	427,572	0	G	A	100.0%	111.8 / NA	31	D96N (GAC→AAC)	yajC	Sec translocon accessory complex subunit YajC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/10);  total (21/10)

GGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTG  >  NC_000913/427494‑427649                                                                               |                                                                              ggtCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAAc                                                                             >  2:93989/1‑81 (MQ=255)    cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTc                                                                          >  1:93984/1‑81 (MQ=255)     gcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCg                                                                         >  1:93983/1‑81 (MQ=255)      cgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGt                                                                        <  1:93948/81‑1 (MQ=255)       gTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCg                                                                         >  2:93993/1‑79 (MQ=255)       gTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCg                                                                         >  1:93978/1‑79 (MQ=255)       gTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCg                                                                         >  1:93985/1‑79 (MQ=255)              aaGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCg                                                                >  2:93979/1‑81 (MQ=255)                 tAGCGGAAAACGGCTACATTGCTATCGCGCTAAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTcc                                                             <  2:93949/81‑1 (MQ=255)                      gAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCg                                                        <  2:93950/81‑1 (MQ=255)                       aaaaCGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCg                                                        >  1:93976/1‑80 (MQ=255)                           cGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAgg                                                   <  1:93951/81‑1 (MQ=255)                            ggCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAgg                                                   >  2:93975/1‑80 (MQ=255)                                 cATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCAcc                                               <  2:93952/79‑1 (MQ=255)                                     gCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAg                                         >  2:93994/1‑81 (MQ=255)                                      cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAgg                                        >  2:93987/1‑81 (MQ=255)                                       tATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGgc                                       >  1:93982/1‑81 (MQ=255)                                           gcgcTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCt                                    <  2:93953/80‑1 (MQ=255)                                            cgcTGAATGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGt                                  <  2:93954/81‑1 (MQ=255)                                                  aTGACACCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTaa                            <  2:93955/81‑1 (MQ=255)                                                     acacCACTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAAt                         >  2:93998/1‑81 (MQ=255)                                                      caccacTGAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAAtt                        >  1:93990/1‑81 (MQ=255)                                                             gAAGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTccc                  >  2:94001/1‑80 (MQ=255)                                                              aaGTAGTTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCt                 >  2:94005/1‑80 (MQ=255)                                                                gtagtTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAg              >  2:94013/1‑81 (MQ=255)                                                                  agtTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAgg             >  1:94006/1‑80 (MQ=255)                                                                   gtTATTAAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGa           <  1:93956/81‑1 (MQ=255)                                                                     tattaAACGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAAt         >  1:94003/1‑81 (MQ=255)                                                                            cGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCgtg  >  2:93999/1‑81 (MQ=255)                                                                            cGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCgtg  >  2:93992/1‑81 (MQ=255)                                                                            cGTAACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCgtg  <  1:93957/81‑1 (MQ=255)                                                                               |                                                                              GGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTG  >  NC_000913/427494‑427649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑