Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,290,611	G→A	G46S (GGC→AGC)	nrfD →	putative menaquinol‑cytochrome c reductase subunit NrfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,290,611	0	G	A	100.0%	153.1 / NA	43	G46S (GGC→AGC)	nrfD	putative menaquinol‑cytochrome c reductase subunit NrfD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/14);  total (29/14)

ATCTACCTGTTTTTGATTGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGGGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGATCCTCGGTCTGTTGATCCTC  >  NC_000913/4290533‑4290688                                                                               |                                                                              aTCTACCTGTTTTTGATTGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAggcgagc                                                                             <  1:859123/81‑1 (MQ=255) aTCTACCTGTTTTTGATTGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAggcgagc                                                                             >  1:859151/1‑81 (MQ=255)     aCCTGTTTTTGATTGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTg                                                                         >  1:859156/1‑81 (MQ=255)      ccTGTTTTTGATTGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAggcgagcg                                                                            <  1:859124/77‑1 (MQ=255)         gTTTTTGATTGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGa                                                                     >  1:859194/1‑81 (MQ=255)            tttGATTGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAg                                                                  >  2:859163/1‑81 (MQ=255)             ttGATTGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAg                                                                  >  2:859162/1‑80 (MQ=255)             ttGATTGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGCCGAGCGCTGCAGCCAg                                                                  >  2:859169/1‑80 (MQ=255)                  tGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGtt                                                            >  1:859146/1‑81 (MQ=255)                    gTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTtgc                                                          >  1:859181/1‑81 (MQ=255)                    gTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTtg                                                           <  1:859125/80‑1 (MQ=255)                       tttCTGCTGGTCTGGTGACGCTGGCCGTGCTATTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTtgctgc                                                       <  2:859126/81‑1 (MQ=255)                           tgctgGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGcac                                                   >  2:859172/1‑81 (MQ=255)                             ctggtctggtGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGcacca                                                 >  2:859202/1‑81 (MQ=255)                             ctggtctggtGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGcacca                                                 >  2:859160/1‑81 (MQ=255)                             ctggtctggtGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGcacc                                                  <  2:859127/80‑1 (MQ=255)                               ggtctggtGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACg                                               >  1:859179/1‑81 (MQ=255)                                gtctggtGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGc                                              <  1:859128/81‑1 (MQ=255)                                 tctggtGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCt                                             <  2:859129/81‑1 (MQ=255)                                  ctggtGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCt                                             >  1:859204/1‑80 (MQ=255)                                   tggtGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGa                                           >  1:859174/1‑81 (MQ=255)                                     gtGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGAtt                                         >  1:859178/1‑81 (MQ=255)                                     gtGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGAtt                                         <  1:859130/81‑1 (MQ=255)                                       gACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGt                                       >  1:859185/1‑81 (MQ=255)                                       gACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGt                                       >  2:859184/1‑81 (MQ=255)                                        aCGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGt                                       <  2:859131/80‑1 (MQ=255)                                         cGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTcg                                     >  1:859197/1‑81 (MQ=255)                                          gCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTcgg                                    >  1:859191/1‑81 (MQ=255)                                          gCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTcgg                                    >  1:859176/1‑81 (MQ=255)                                             ggCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTcgggcc                                 >  1:859195/1‑81 (MQ=255)                                             ggCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTcgggcc                                 >  2:859168/1‑81 (MQ=255)                                                 gTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTcgggccgggc                             >  1:859196/1‑81 (MQ=255)                                                 gTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTcgggccgggc                             >  2:859177/1‑81 (MQ=255)                                                 gTGCTGTTACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTcgggccgggc                             >  2:859153/1‑81 (MQ=255)                                                       ttACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTg                       <  1:859132/81‑1 (MQ=255)                                                        tACGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGa                      >  2:859193/1‑81 (MQ=255)                                                          cGTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGAt                     <  2:859133/80‑1 (MQ=255)                                                           gTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGATcc                   >  1:859189/1‑81 (MQ=255)                                                           gTCGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGATcc                   >  1:859186/1‑81 (MQ=255)                                                             cGCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCATGCTGATTGTCGGGCCGGGCGCGGTGATCCTc                 >  2:859228/1‑81 (MQ=255)                                                              gCTTCTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGATCCTc                 <  2:859134/80‑1 (MQ=255)                                                                  cTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGATCCTCGGt              <  1:859136/79‑1 (MQ=255)                                                                  cTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGATCCTCGGTc             <  2:859137/80‑1 (MQ=255)                                                                  cTACCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGATCCTCGGTc             <  1:859135/80‑1 (MQ=255)                                                                    aCCCGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGATCCTCGGTCTGt          >  2:859175/1‑81 (MQ=255)                                                                      ccGCAGGCGAGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGATCCTCGGTCTGTTg        <  1:859138/81‑1 (MQ=255)                                                                            gcgagcggTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGATCCTCGGTCTGTTGATCCTc  >  1:859201/1‑81 (MQ=255)                                                                               |                                                                              ATCTACCTGTTTTTGATTGGTATTTCTGCTGGTCTGGTGACGCTGGCCGTGCTGTTACGTCGCTTCTACCCGCAGGCGGGCGGTGCAGACAGTACGTTGCTGCGCACCACGCTGATTGTCGGGCCGGGCGCGGTGATCCTCGGTCTGTTGATCCTC  >  NC_000913/4290533‑4290688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑