Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,308,436	A→C	F15V (TTT→GTT)	alsE ←	D‑allulose‑6‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,308,436	0	A	C	100.0%	106.4 / NA	30	F15V (TTT→GTT)	alsE	D‑allulose‑6‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/16);  total (14/16)

GGGACAAAGTGACCGTCCATGATATCGATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTCAATTCCTTACTTACTGATAAGACGGT  >  NC_000913/4308360‑4308508                                                                             |                                                                         gggACAAAGTGACCGTCCATGATATCGATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACttt                                                                       <  1:863620/80‑1 (MQ=255) gggACAAAGTGACCGTCCATGATATCGATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTc                                                                      <  1:863619/81‑1 (MQ=255)    aCAAAGTGACCGTCCATGATATCGATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTcagc                                                                   <  2:863621/81‑1 (MQ=255)      aaaGTGACCGTCCATGATATCGATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTcag                                                                    >  2:863661/1‑78 (MQ=255)        aGTGACCGTCCATGATATCGATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGAt                                                               <  1:863622/81‑1 (MQ=255)          tGACCGTCCATGATATCGATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATcc                                                             <  1:863623/81‑1 (MQ=255)           gACCGTCCATGATATCGATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCa                                                            >  2:863656/1‑81 (MQ=255)              cGTCCATGATATCGATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATac                                                         <  1:863624/81‑1 (MQ=255)                  cATGATATCGATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATAcaca                                                      <  1:863625/80‑1 (MQ=255)                     gATATCGATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTaa                                                  >  2:863666/1‑81 (MQ=255)                           gATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAggg                                            >  1:863668/1‑81 (MQ=255)                             tgtgGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAgggg                                           >  2:863664/1‑80 (MQ=255)                                 gAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGgaga                                       <  2:863626/80‑1 (MQ=255)                                     tAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGAtttt                                   >  2:863686/1‑80 (MQ=255)                                     tAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTc                                  <  2:863627/81‑1 (MQ=255)                                      aaTCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCa                                 >  2:863683/1‑81 (MQ=255)                                         cGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGt                              >  1:863674/1‑81 (MQ=255)                                           gCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGtt                             >  1:863678/1‑80 (MQ=255)                                           gCATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTc                            <  2:863628/81‑1 (MQ=255)                                            cATGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTc                            >  1:863665/1‑80 (MQ=255)                                             aTGGCTGTCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTCaa                          >  1:863673/1‑81 (MQ=255)                                                    tCGATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTCAATTCctt                    <  2:863629/80‑1 (MQ=255)                                                      gATAAATTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTCAATTCcttact                 <  1:863630/81‑1 (MQ=255)                                                           aTTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTCAATTCCTtacttact             >  2:863682/1‑80 (MQ=255)                                                           aTTCGATCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTCAATTCCTTACTTACTg            <  1:863631/81‑1 (MQ=255)                                                                aTCTGTTCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTCAATTCCTTACTTACTGAt          >  2:863675/1‑78 (MQ=255)                                                                     ttCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTCAATTCCTTACTTACTGATAAg       <  1:863633/76‑1 (MQ=255)                                                                     ttCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTCAATTCCTTACTTACTGATAAGAc     <  1:863634/78‑1 (MQ=255)                                                                     ttCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTCAATTCCTTACTTACTGATAAGACg    <  2:863632/79‑1 (MQ=255)                                                                     ttCTTTAACTTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTCAATTCCTTACTTACTGATAAGACGGt  >  2:863685/1‑81 (MQ=255)                                                                             |                                                                         GGGACAAAGTGACCGTCCATGATATCGATGTGGAAGTAATCGGCATGGCTGTCGATAAATTCGATCTGTTCTTTAAATTTCAGCAGATCCATACACATTAACGAGGGGGAGATTTTCATGTTCAATTCCTTACTTACTGATAAGACGGT  >  NC_000913/4308360‑4308508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑