Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,265	C→T	E243K (GAA→AAA)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,265	0	C	T	100.0%	50.8 / NA	15	E243K (GAA→AAA)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/6);  total (9/6)

AAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACA  >  NC_000913/4312199‑4312334                                                                   |                                                                      aaaaaGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTTCGGGGCTGGTGAGc                                                         <  2:864425/81‑1 (MQ=255) aaaaaGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTTCGGGGCTGGTGAGc                                                         <  2:864426/81‑1 (MQ=255)       cGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTTCGGGGCTGGTGAGCAaataa                                                   >  1:864457/1‑81 (MQ=255)            aGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTTCGGGGCTGGTGAGCAAataatataa                                              >  1:864476/1‑81 (MQ=255)                  gttagttGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTTCGGGGCTGGTGAGCAAATAATATAATCGGc                                         >  1:864468/1‑80 (MQ=255)                      gttGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTTCGGGGCTGGTGAGCAAATAATATAATCGGCCAGtt                                    <  2:864427/81‑1 (MQ=255)                           aaTATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTTCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGc                                >  1:864454/1‑80 (MQ=255)                            atatTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTTCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCt                               <  2:864428/80‑1 (MQ=255)                                      cGAGGCATTACGACCTAATAACGGCGTTTTCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATg                    >  2:864465/1‑81 (MQ=255)                                       gAGGCATTACGACCTAATAACGGCGTTTTCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATgg                   <  2:864429/81‑1 (MQ=255)                                        aGGCATTACGACCTAATAACGGCGTTTTCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATgg                   >  2:864459/1‑80 (MQ=255)                                            aTTACGACCTAATAACGGCGTTTTCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGc               >  1:864456/1‑80 (MQ=255)                                                   ccTAATAACGGCGTTTTCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGtt       <  1:864430/81‑1 (MQ=255)                                                         aaCGGCGTTTTCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACa  >  1:864451/1‑80 (MQ=255)                                                         aaCGGCGTTTTCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACa  >  2:864471/1‑80 (MQ=255)                                                                   |                                                                      AAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACA  >  NC_000913/4312199‑4312334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑