Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,368,325	C→T	M1M (ATG→ATA) †	aspA ←	aspartate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,368,325	0	C	T	100.0%	104.0 / NA	29	M1M (ATG→ATA) †	aspA	aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/17);  total (12/17)

TGAACACCATAGTAGGCATCAGCTGGAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGACATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTT  >  NC_000913/4368251‑4368398                                                                           |                                                                          tgAACACCATAGTAGGCATCAGCTGGAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATAtgtg                                                                      <  1:878106/80‑1 (MQ=255) tgAACACCATAGTAGGCATCAGCTGGAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATAtgtg                                                                      <  2:878105/80‑1 (MQ=255)      acCATAGTAGGCATCAGCTGGAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACctt                                                                >  1:878139/1‑81 (MQ=255)         aTAGTAGGCATCAGCTGGAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACcttctt                                                             <  2:878107/81‑1 (MQ=255)          tagtagGCATCAGCTGGAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCttt                                                            <  1:878108/81‑1 (MQ=255)            gtagGCATCAGCTGGAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCttttt                                                          <  2:878110/81‑1 (MQ=255)            gtagGCATCAGCTGGAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCtttt                                                           <  1:878109/80‑1 (MQ=255)             tagGCATCAGCTGGAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTc                                                         <  2:878111/81‑1 (MQ=255)                  aTCAGCTGGAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCt                                                    >  1:878148/1‑81 (MQ=255)                  aTCAGCTGGAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCt                                                    >  1:878156/1‑81 (MQ=255)                          gAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGa                                            >  1:878142/1‑81 (MQ=255)                             cTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGAttt                                         <  1:878112/81‑1 (MQ=255)                                    ggTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTa                                   >  1:878145/1‑80 (MQ=255)                                    ggTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTa                                   >  2:878151/1‑80 (MQ=255)                                      tACCCAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAac                                <  2:878113/81‑1 (MQ=255)                                          cAACAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAacacac                            <  2:878114/81‑1 (MQ=255)                                           aaCAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAGacacac                            >  2:878152/1‑80 (MQ=255)                                            aCAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAacaca                             >  1:878154/1‑78 (MQ=255)                                             cAGATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGa                         <  2:878115/81‑1 (MQ=255)                                               gATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGaa                        >  2:878155/1‑80 (MQ=255)                                               gATCTTCTTCGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAat                       <  1:878116/81‑1 (MQ=255)                                                        cGATACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGt              >  2:878150/1‑81 (MQ=255)                                                           tACGAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACGCACAGAATATATGTGGTTTc           <  2:878117/81‑1 (MQ=255)                                                              gAATGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGaa        >  2:878146/1‑81 (MQ=255)                                                               aaTGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAAt       <  2:878118/81‑1 (MQ=255)                                                                aTGTTGTTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATg      <  1:878119/81‑1 (MQ=255)                                                                 tgttgtTTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGt     >  2:878158/1‑81 (MQ=255)                                                                   ttgttTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGtt    <  2:878120/80‑1 (MQ=255)                                                                     gttTGATATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGtttt  <  2:878121/80‑1 (MQ=255)                                                                           |                                                                          TGAACACCATAGTAGGCATCAGCTGGAACTTCCCTGGTACCCAACAGATCTTCTTCGATACGAATGTTGTTTGACATGTGAACCTTCTTTTTCAAGCTGCCAATGATTTGCTTTAAACACACAGAATATATGTGGTTTCGAATGTTTT  >  NC_000913/4368251‑4368398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑