Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	430,613	C→T	I3I (ATC→ATT)	yajD →	HNH nuclease family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	430,613	0	C	T	100.0%	71.3 / NA	21	I3I (ATC→ATT)	yajD	HNH nuclease family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/11);  total (10/11)

GGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTC  >  NC_000913/430536‑430689                                                                              |                                                                             ggAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTattatc                                                                           >  2:94657/1‑81 (MQ=255)         cGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCaaaaaa                                                                   >  2:94666/1‑81 (MQ=255)                  ccGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAACACTATGcgcg                                                          <  2:94632/81‑1 (MQ=255)                   cGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCgg                                                         >  1:94660/1‑81 (MQ=255)                       tCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGt                                                        >  2:94652/1‑78 (MQ=255)                            ggAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGc                                                <  1:94633/81‑1 (MQ=255)                                aCTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCt                                            <  1:94634/81‑1 (MQ=255)                                 cTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTa                                           <  1:94635/81‑1 (MQ=255)                                    cgcgTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATc                                         <  2:94636/80‑1 (MQ=255)                                     gcgTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATcgc                                       <  2:94637/81‑1 (MQ=255)                                     gcgTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATcgc                                       >  2:94659/1‑81 (MQ=255)                                     gcgTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATcgc                                       >  2:94662/1‑81 (MQ=255)                                       gTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATcgcg                                      >  1:94661/1‑80 (MQ=255)                                         aaCCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaa                                    >  1:94654/1‑80 (MQ=255)                                          aCCCTACATTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaaaa                                  <  2:94638/81‑1 (MQ=255)                                                 aTTTCATCCAGGTAACTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCAtt                            >  1:94664/1‑80 (MQ=255)                                                              aaCTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGt              >  1:94672/1‑81 (MQ=255)                                                               aCTTTTCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGt              <  2:94639/80‑1 (MQ=255)                                                                  tttCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGt          >  1:94670/1‑81 (MQ=255)                                                                   ttCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGt          <  2:94640/80‑1 (MQ=255)                                                                    tCATGGCTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTc         <  2:94641/80‑1 (MQ=255)                                                                          cTATTATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTc  <  2:94642/81‑1 (MQ=255)                                                                              |                                                                             GGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTC  >  NC_000913/430536‑430689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑