Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,460,452	G→A	intergenic (‑88/+70)	nrdG ← / ← nrdD	anaerobic ribonucleoside‑triphosphate reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,460,452	0	G	A	100.0%	137.9 / NA	38	intergenic (‑88/+70)	nrdG/nrdD	anaerobic ribonucleoside‑triphosphate reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/23);  total (15/23)

GCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCT  >  NC_000913/4460376‑4460532                                                                             |                                                                                 gCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACaa                                                                               <  2:900472/80‑1 (MQ=255) gCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACa                                                                                <  1:900474/79‑1 (MQ=255) gCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAAt                                                                              <  2:900473/81‑1 (MQ=255)  cTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAAtt                                                                             <  1:900475/81‑1 (MQ=255)    tATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGc                                                                           >  1:900516/1‑81 (MQ=255)     aTCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGc                                                                           <  2:900476/80‑1 (MQ=255)      tCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGcc                                                                          <  2:900477/80‑1 (MQ=255)        aGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCaaa                                                                       >  1:900522/1‑81 (MQ=255)         gTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAAt                                                                      <  2:900478/81‑1 (MQ=255)             gcgTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAg                                                                  <  2:900479/81‑1 (MQ=255)             gcgTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAg                                                                  >  1:900514/1‑81 (MQ=255)             gcgTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAg                                                                  >  1:900517/1‑81 (MQ=255)               gtgtAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGc                                                                <  1:900480/81‑1 (MQ=255)                  tAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCgg                                                             <  2:900481/81‑1 (MQ=255)                   aGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCTGACCTGGAACAATTGCCAAATGTAGGCCGGa                                                            >  2:900527/1‑81 (MQ=255)                   aGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGa                                                            >  1:900513/1‑81 (MQ=255)                   aGGCTGCATCCGGTAATCAATGACTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGa                                                            >  1:900512/1‑81 (MQ=255)                         cATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAgg                                                       <  2:900482/80‑1 (MQ=255)                         cATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGc                                                      <  2:900483/81‑1 (MQ=255)                          aTCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCg                                                     >  1:900524/1‑81 (MQ=255)                           tCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGt                                                    <  2:900485/81‑1 (MQ=255)                           tCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGt                                                    <  2:900484/81‑1 (MQ=255)                             cGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGtt                                                   <  1:900486/80‑1 (MQ=255)                                 aaTCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTAcgc                                              >  2:900543/1‑81 (MQ=255)                                  aTCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTAcgc                                              <  1:900487/80‑1 (MQ=255)                                      aTGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCAt                                         <  1:900488/81‑1 (MQ=255)                                      aTGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCAt                                         <  2:900489/81‑1 (MQ=255)                                       tGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATc                                        <  1:900490/81‑1 (MQ=255)                                             aTGCGACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAt                                  <  2:900491/81‑1 (MQ=255)                                                 gACGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGc                              >  1:900532/1‑81 (MQ=255)                                                  aCGCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCt                             >  1:900519/1‑81 (MQ=255)                                                    gCTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCt                             >  1:900521/1‑79 (MQ=255)                                                     cTGTCGCGTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAg                          >  1:900520/1‑81 (MQ=255)                                                          gcgTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                        >  2:900525/1‑78 (MQ=255)                                                          gcgTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                      <  1:900492/80‑1 (MQ=255)                                                           cgTCTTATCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                      >  1:900530/1‑79 (MQ=255)                                                                 aTCAGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTg                 <  2:900493/78‑1 (MQ=255)                                                                    aGACCTGGAACAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGAtt           <  1:900494/81‑1 (MQ=255)                                                                             gCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCt  <  1:900496/81‑1 (MQ=255)                                                                             |                                                                                 GCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCT  >  NC_000913/4460376‑4460532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑