Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,523	G→A	W29* (TGG→TGA)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,523	0	G	A	100.0%	61.3 / NA	18	W29* (TGG→TGA)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/10);  total (8/10)

AATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATT  >  NC_000913/4475445‑4475600                                                                               |                                                                              aaTATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACg                                                                             >  1:904113/1‑81 (MQ=255)   tatCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACgtg                                                                           <  2:904089/81‑1 (MQ=255)       aGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTg                                                                        <  2:904090/80‑1 (MQ=255)                        aaCACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGccc                                                      <  1:904091/81‑1 (MQ=255)                         acacATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCg                                                     >  1:904108/1‑81 (MQ=255)                                acattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGcc                                              >  2:904115/1‑81 (MQ=255)                                 cattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGcc                                              <  2:904092/80‑1 (MQ=255)                                   ttaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCggg                                           >  2:904109/1‑81 (MQ=255)                                            aaTTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGAt                                  <  1:904093/81‑1 (MQ=255)                                            aaTTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGAt                                  <  1:904094/81‑1 (MQ=255)                                             aTTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGAt                                  >  1:904118/1‑80 (MQ=255)                                               tttAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATaa                                >  1:904110/1‑80 (MQ=255)                                                 tAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATaa                                >  1:904117/1‑78 (MQ=255)                                                  aaaCAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAg                             <  1:904095/80‑1 (MQ=255)                                                                aGTTTTAAATACCTGACGTGTTGGAATGAATAATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATAttctttc              <  2:904096/81‑1 (MQ=255)                                                                       aaTACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTgg        <  1:904097/80‑1 (MQ=255)                                                                           ccTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAt   >  2:904124/1‑81 (MQ=255)                                                                            cTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAtt  <  2:904098/81‑1 (MQ=255)                                                                               |                                                                              AATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATT  >  NC_000913/4475445‑4475600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑