Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,503,704	A→G	intergenic (‑273/‑354)	insG ← / → yjhB	KpLE2 phage‑like element; IS4 putative transposase/putative sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,503,704	0	A	G	100.0%	54.9 / NA	16	intergenic (‑273/‑354)	insG/yjhB	KpLE2 phage‑like element; IS4 putative transposase/putative sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/6);  total (10/6)

TAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCA  >  NC_000913/4503637‑4503771                                                                    |                                                                    taAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCttt                                                        <  1:910409/81‑1 (MQ=255) taAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCttt                                                        >  1:910438/1‑81 (MQ=255)    aGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCt                                                     >  2:910442/1‑81 (MQ=255)     ggTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTc                                                    <  1:910410/81‑1 (MQ=255)        ataCAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCAtt                                                 >  2:910435/1‑81 (MQ=255)         taCAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCAttt                                                <  1:910411/81‑1 (MQ=255)         taCAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCAtt                                                 <  1:910412/80‑1 (MQ=255)            aGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGAc                                             <  1:910413/81‑1 (MQ=255)            aGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGAc                                             >  2:910445/1‑81 (MQ=255)             gAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGAc                                             >  1:910437/1‑80 (MQ=255)              aaaaGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACg                                            >  1:910433/1‑80 (MQ=255)                  gCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAgg                                        >  1:910441/1‑80 (MQ=255)                                         aCAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGAc                >  2:910439/1‑81 (MQ=255)                                             cTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTAc             >  1:910447/1‑80 (MQ=255)                                             cTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACg            <  2:910414/81‑1 (MQ=255)                                                        gCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGca  >  1:910436/1‑80 (MQ=255)                                                                    |                                                                    TAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCA  >  NC_000913/4503637‑4503771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑