Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,505,023	T→C	G322G (GGT→GGC)	yjhB →	putative sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,505,023	0	T	C	100.0%	46.8 / NA	14	G322G (GGT→GGC)	yjhB	putative sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/10);  total (4/10)

CTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATT  >  NC_000913/4504947‑4505098                                                                             |                                                                            ctctTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGCATTg                                                                         <  1:910577/81‑1 (MQ=255)         tttATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGCATTGCAGggtt                                                                  <  2:910578/80‑1 (MQ=255)             tttCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGCATTGCAGggttggtt                                                              <  1:910579/80‑1 (MQ=255)               tCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGCATTGCAGGGTTGGTTcc                                                            <  1:910581/80‑1 (MQ=255)               tCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGCATTGCAGGGTTGGTTcc                                                            <  2:910580/80‑1 (MQ=255)                   tgAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGCATTGCAGGGTTGGTTCCaaaa                                                        <  1:910582/80‑1 (MQ=255)                     aaaaaCTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGCATTGCAGGGTTGGTTCCAAAAtt                                                      <  1:910583/80‑1 (MQ=255)                       aaaCTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGCATTGCAGGGTTGGTTCCAAAATTta                                                    >  1:910600/1‑80 (MQ=255)                        aaCTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGCATTGCAGGGTTGGTTCCAAAATTtata                                                  >  1:910615/1‑81 (MQ=255)                               ctcttATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGCATTGCAGGGTTGGTTCCAAAATTTATATATGAt                                            <  1:910584/80‑1 (MQ=255)                                                       gATTAATGTTTACAAATTTAGGCATTGCAGGGTTGGTTCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGa                    <  2:910585/80‑1 (MQ=255)                                                                ttACAAATTTAGGCATTGCAGGGTTGGTTCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCg          >  1:910598/1‑81 (MQ=255)                                                                       tttAGGCATTGCAGGGTTGGTTCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCttat   <  2:910586/81‑1 (MQ=255)                                                                         tAGGCATTGCAGGGTTGGTTCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCttatt  >  2:910604/1‑80 (MQ=255)                                                                             |                                                                            CTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATT  >  NC_000913/4504947‑4505098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑