Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,506,209	C→T	A308V (GCC→GTC)	yjhC →	KpLE2 phage‑like element; putative oxidoreductase YjhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,506,209	0	C	T	100.0%	103.1 / NA	29	A308V (GCC→GTC)	yjhC	KpLE2 phage‑like element; putative oxidoreductase YjhC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/12);  total (17/12)

GGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑AAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGATTATATTGA  >  NC_000913/4506136‑4506284                                                                           |                                                                            ggCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGttt                                                                       <  2:910725/81‑1 (MQ=255)    aaTATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTtaa                                                                     >  1:910768/1‑80 (MQ=255)                  aaTGGATGGCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGt                                                      >  1:910771/1‑81 (MQ=255)                        tggCGCTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATtcc                                                >  1:910778/1‑81 (MQ=255)                          gcgcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATtcctc                                              >  2:910787/1‑81 (MQ=255)                          gcgcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATtcct                                               <  2:910726/80‑1 (MQ=255)                           cgcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATtcctcc                                             <  1:910727/81‑1 (MQ=255)                            gcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCa                                            >  2:910777/1‑81 (MQ=255)                            gcTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCa                                            >  1:910767/1‑81 (MQ=255)                             cTATAGCATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCat                                           <  1:910728/81‑1 (MQ=255)                                   cATATGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATc                                     <  1:910729/81‑1 (MQ=255)                                       tGGTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCctc                                  <  2:910730/80‑1 (MQ=255)                                         gTCATCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCtgt                               >  2:910773/1‑81 (MQ=255)                                            aTCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTgtggt                            >  1:910779/1‑81 (MQ=255)                                            aTCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTgtggt                            >  2:910782/1‑81 (MQ=255)                                            aTCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTgtgg                             <  1:910731/80‑1 (MQ=255)                                             tCCAGGAAAAAAAAACACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTgtggtg                           >  1:910732/1‑81 (MQ=255)                                                      aaaaaaCACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTg                  <  1:910733/81‑1 (MQ=255)                                                       aaaaaCACAATTATGGCTTGTAAGTTTAATAGGAAAGGAGACGTTATTCCT‑CATAATATCCTCTGTGGTGCAAAACCTgaa                <  2:910734/81‑1 (MQ=255)                                                         aaaCACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTg                  >  1:910793/1‑78 (MQ=255)                                                          aaCACCATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTgaag               >  2:910774/1‑80 (MQ=255)                                                              ccATTATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGAt          <  2:910735/81‑1 (MQ=255)                                                                  tATGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGATtata      >  1:910784/1‑81 (MQ=255)                                                                   aTGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGATTatat     >  1:910785/1‑81 (MQ=255)                                                                    tGGCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGATTATAtt    <  1:910736/81‑1 (MQ=255)                                                                     ggCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGATTATAtt    >  1:910780/1‑80 (MQ=255)                                                                     ggCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGATTATAtt    >  2:910775/1‑80 (MQ=255)                                                                      gCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGATTATATTg   <  2:910737/80‑1 (MQ=255)                                                                      gCTTGTCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGATTATATTGa  >  1:910781/1‑81 (MQ=255)                                                                           |                                                                            GGCAATATGACCTCAGAAATGGATGGCGCTATAGCATATGGTCATCCAGG‑AAAAAAAACACCATTATGGCTTGCCAGTTTAATAAGAAAGGAGACGTTATTCCTCCATAATATCCTCTGTGGTGCAAAACCTGAAGAAGATTATATTGA  >  NC_000913/4506136‑4506284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑