Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	443,213	G→A	R143R (CGC→CGT)	yajL ←	protein/nucleic acid deglycase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	443,213	0	G	A	100.0%	108.5 / NA	30	R143R (CGC→CGT)	yajL	protein/nucleic acid deglycase 3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/14);  total (16/14)

ATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAATCGG  >  NC_000913/443139‑443284                                                                           |                                                                        aTTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCtt                                                                    <  1:97400/80‑1 (MQ=255) aTTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTg                                                                   >  1:97430/1‑81 (MQ=255)  ttttCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGt                                                                  <  1:97401/81‑1 (MQ=255)       aGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAg                                                              <  2:97402/80‑1 (MQ=255)          ccAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCAt                                                          <  2:97403/81‑1 (MQ=255)            aaaGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTg                                                        >  2:97433/1‑81 (MQ=255)              aGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGtt                                                      <  2:97404/81‑1 (MQ=255)               gTCGATAGCTGTACACGGCCCCTGGCTGGTCAGAAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTc                                                     <  1:97405/81‑1 (MQ=255)                 cGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTcgg                                                   >  1:97443/1‑81 (MQ=255)                  gATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTcggc                                                  >  1:97434/1‑81 (MQ=255)                   aTAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTcggcg                                                 >  2:97436/1‑81 (MQ=255)                      gCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCggg                                               >  2:747799/1‑80 (MQ=255)                        tGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAAt                                            >  1:97453/1‑81 (MQ=255)                           aCCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAAtttt                                         >  2:97439/1‑81 (MQ=255)                           aCCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAAtttt                                         >  1:97452/1‑81 (MQ=255)                               ggCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCt                                     >  2:97448/1‑81 (MQ=255)                                 ccccTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCttt                                   >  2:97507/1‑81 (MQ=255)                                 ccccTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCttt                                   <  1:97406/81‑1 (MQ=255)                                  cccTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTc                                  <  2:97408/81‑1 (MQ=255)                                  cccTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTc                                  <  1:97407/81‑1 (MQ=255)                                   ccTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCttt                                   >  2:97441/1‑79 (MQ=255)                                        ctggTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTc                            <  1:97409/81‑1 (MQ=255)                                         tggTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCg                           >  2:97449/1‑81 (MQ=255)                                          ggTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCgg                          >  2:97446/1‑81 (MQ=255)                                           gTCAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCggg                         <  1:97410/81‑1 (MQ=255)                                             cAGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCggg                         >  2:97492/1‑79 (MQ=255)                                              aGCAATTTTACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGaa                       <  1:97411/80‑1 (MQ=255)                                                     ttACCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTc                <  1:97412/80‑1 (MQ=255)                                                       aCCCGTGCATCCCAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCat              <  1:97413/80‑1 (MQ=255)                                                                  ccAGACGACACGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAATCgg  >  1:97464/1‑81 (MQ=255)                                                                           |                                                                        ATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAATCGG  >  NC_000913/443139‑443284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑