Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,527,240	T→A	T100S (ACT→TCT)	sgcE ←	KpLE2 phage‑like element; putative epimerase SgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,527,240	0	T	A	100.0%	160.8 / NA	44	T100S (ACT→TCT)	sgcE	KpLE2 phage‑like element; putative epimerase SgcE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (20/24);  total (20/24)

AGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTCAGCCAGCGCGTT  >  NC_000913/4527164‑4527315                                                                             |                                                                            aGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAAc                                                                         <  1:915720/81‑1 (MQ=255)  gATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACg                                                                        >  2:915792/1‑81 (MQ=255)    tatCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGtt                                                                      <  2:915721/81‑1 (MQ=255)      tCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGttt                                                                     <  2:915722/80‑1 (MQ=255)      tCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTc                                                                    >  2:915860/1‑81 (MQ=255)        gCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTcc                                                                   <  1:915724/80‑1 (MQ=255)        gCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTcc                                                                   >  2:915789/1‑80 (MQ=255)        gCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCg                                                                  <  2:915723/81‑1 (MQ=255)         ccAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCg                                                                  >  2:915776/1‑80 (MQ=255)           aGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAg                                                               >  1:915784/1‑81 (MQ=255)               gTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATa                                                           <  2:915725/81‑1 (MQ=255)                  gATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGt                                                         <  1:915726/80‑1 (MQ=255)                   aTCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTcc                                                       <  2:915727/81‑1 (MQ=255)                    tCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTc                                                        >  2:915794/1‑79 (MQ=255)                      ggggTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGc                                                    >  1:915804/1‑81 (MQ=255)                        ggTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGCATGGAGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCgc                                                  >  1:915782/1‑80 (MQ=255)                         gTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGtt                                                 <  1:915728/81‑1 (MQ=255)                          ttGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGttt                                                >  1:915790/1‑81 (MQ=255)                                gATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCAt                                          <  1:915729/81‑1 (MQ=255)                                   tAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGaa                                       >  1:915796/1‑81 (MQ=255)                                     aaTACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAAc                                      <  2:915730/80‑1 (MQ=255)                                         ccAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGa                                 <  2:915731/81‑1 (MQ=255)                                          cAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAt                                <  2:915732/81‑1 (MQ=255)                                            gTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATcc                              <  1:915733/81‑1 (MQ=255)                                               cGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCAcg                           <  2:915734/81‑1 (MQ=255)                                               cGGCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCAcg                           >  2:915799/1‑81 (MQ=255)                                                 gCTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCAcgcg                         >  2:915821/1‑81 (MQ=255)                                                  cTCGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCgg                        >  2:915797/1‑81 (MQ=255)                                                    cGTGCGCCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGt                      >  2:915801/1‑81 (MQ=255)                                                       gcgcCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCt                    >  1:915803/1‑80 (MQ=255)                                                        cgcCGGTATGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTg                   <  1:915735/80‑1 (MQ=255)                                                               aTGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTcagc           >  1:915811/1‑81 (MQ=255)                                                               aTGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTcagc           >  2:915802/1‑81 (MQ=255)                                                                tGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTcagcc          <  1:915737/81‑1 (MQ=255)                                                                tGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTcagc           >  1:915805/1‑80 (MQ=255)                                                                tGGCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTcagc           <  2:915736/80‑1 (MQ=255)                                                                  gCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTcagccag        <  2:915739/81‑1 (MQ=255)                                                                  gCGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTcagccag        <  1:915738/81‑1 (MQ=255)                                                                   cGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTcagccagc       <  1:915741/81‑1 (MQ=255)                                                                   cGGATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTcagccagc       <  2:915740/81‑1 (MQ=255)                                                                    ggATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTCAGCCAgcg      >  1:915812/1‑81 (MQ=255)                                                                     gATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTCAGCCAgcgc     <  2:915743/81‑1 (MQ=255)                                                                     gATCTCAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTCAGCCAgcgc     <  2:915742/81‑1 (MQ=255)                                                                         tcAGATAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTCAGCCAGCGCGtt  >  2:915843/1‑80 (MQ=255)                                                                             |                                                                            AGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTCAGCCAGCGCGTT  >  NC_000913/4527164‑4527315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑