Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,536,178	A→G	intergenic (+147/+436)	yjhR → / ← nanS	KpLE2 phage‑like element; PLD‑like domain‑containing protein YjhR/N‑acetyl‑9‑O‑acetylneuraminate esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,536,178	0	A	G	100.0%	42.9 / NA	13	intergenic (+147/+436)	yjhR/nanS	KpLE2 phage‑like element; PLD‑like domain‑containing protein YjhR/N‑acetyl‑9‑O‑acetylneuraminate esterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/6);  total (7/6)

GCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCAT  >  NC_000913/4536115‑4536255                                                                |                                                                              gCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGGTTAATGGCGGGTGCttt                                                              <  2:917745/81‑1 (MQ=255)              ataAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGGTTAATGGCGGGTGCTTTTAGGAATgaaag                                                  >  1:917764/1‑80 (MQ=255)                 aaTCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGGTTAATGGCGGGTTCTTTTAGGAATGATAGaaaa                                              >  2:917763/1‑81 (MQ=255)                 aaTCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGGTTAATGGCGGGTGCTTTTAGGAATgaaagaaa                                               <  1:917746/80‑1 (MQ=255)                           tttACCTAATCATTTTCCTTTGCATAATGCGCCTGGGGTTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCaat                                    <  2:917747/81‑1 (MQ=255)                             tACCTAATCATTTTCCTTTGCATAATGCGCCTGGTGTTAATGGCGGGTTCTTTTTGGAATGAAAGAAAATGCAAGCaataa                                  >  2:917762/1‑81 (MQ=255)                                      aTTTTCCTTTGCATAATGCGCCTGGGGTTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATaataat                         >  2:917774/1‑81 (MQ=255)                                       ttttCCTTTGCATAATGCGCCTGGGGTTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATg                        >  1:917771/1‑81 (MQ=255)                                              ttGCATAATGCGCCTGGGGTTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAAt                 >  2:917773/1‑81 (MQ=255)                                                  aTAATGCGCCTGGGGTTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCt             <  2:917748/81‑1 (MQ=255)                                                   tAATGCGCCTGGGGTTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCtt            <  1:917749/81‑1 (MQ=255)                                                    aaTGCGCCTGGGGTTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCttt           >  1:917766/1‑81 (MQ=255)                                                           cTGGGGTTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAAcatc    <  1:917750/81‑1 (MQ=255)                                                             ggggTTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAAcatcat  >  1:917765/1‑81 (MQ=255)                                                                |                                                                              GCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCAT  >  NC_000913/4536115‑4536255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑