Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,595,481	A→G	V124A (GTT→GCT)	lgoR ←	putative DNA‑binding transcriptional regulator LgoR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,595,481	0	A	G	100.0%	115.4 / NA	32	V124A (GTT→GCT)	lgoR	putative DNA‑binding transcriptional regulator LgoR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/15);  total (17/15)

CATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTA  >  NC_000913/4595405‑4595556                                                                             |                                                                            cATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGcc                                                                           <  1:930116/79‑1 (MQ=255)     cTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCt                                                                      >  1:930156/1‑80 (MQ=255)              ccGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCAGGGCTGACACCcgcg                                                            <  1:930117/81‑1 (MQ=255)                gcgcTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGc                                                          >  1:930165/1‑81 (MQ=255)                  gcTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGccc                                                        >  2:930161/1‑81 (MQ=255)                  gcTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGccc                                                        >  1:930184/1‑81 (MQ=255)                      ttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGa                                                      <  1:930118/79‑1 (MQ=255)                      ttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGc                                                    >  1:930167/1‑81 (MQ=255)                         gCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAg                                                 <  1:930119/81‑1 (MQ=255)                           tgtgAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGtt                                               >  1:930164/1‑81 (MQ=255)                             tgAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTg                                              <  2:930121/80‑1 (MQ=255)                             tgAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGc                                             <  1:930120/81‑1 (MQ=255)                             tgAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGc                                             <  1:930122/81‑1 (MQ=255)                                aTAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGt                                          >  1:930177/1‑81 (MQ=255)                                 tAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGtt                                         <  1:930123/81‑1 (MQ=255)                                   aGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGtt                                         >  2:930178/1‑79 (MQ=255)                                         taACGGCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAAc                                 <  2:930124/81‑1 (MQ=255)                                             ggCCGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGttt                             >  1:930168/1‑81 (MQ=255)                                                cGAATTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTccc                          >  1:930159/1‑81 (MQ=255)                                                  aaTTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCt                        <  1:930125/81‑1 (MQ=255)                                                   aTTTTAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTg                       <  2:930126/81‑1 (MQ=255)                                                    ttttAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTgg                      >  1:930212/1‑81 (MQ=255)                                                       tAAAAGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGc                    <  1:930127/80‑1 (MQ=255)                                                         aaaGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCTAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGt                  >  1:930222/1‑80 (MQ=255)                                                         aaaGATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTa                 <  1:930128/81‑1 (MQ=255)                                                            gATATTCTCTTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACt              >  2:930179/1‑81 (MQ=255)                                                                ttCTCTTACTACAGCCGGGCTGACACCCGCAGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgc           <  2:930129/80‑1 (MQ=255)                                                                  ctctTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCt        >  1:930170/1‑81 (MQ=255)                                                                  ctctTACTACAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCt        >  2:930181/1‑81 (MQ=255)                                                                      tactacAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGtt    >  1:930173/1‑81 (MQ=255)                                                                       actacAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTgg      <  1:930130/78‑1 (MQ=255)                                                                        ctacAGCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTa  >  1:930169/1‑81 (MQ=255)                                                                             |                                                                            CATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTA  >  NC_000913/4595405‑4595556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑