Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,596,220	T→A	D77E (GAT→GAA)	lgoD →	L‑galactonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,596,220	0	T	A	100.0%	51.3 / NA	15	D77E (GAT→GAA)	lgoD	L‑galactonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/3);  total (12/3)

TTTTTTAGTTATCCACGTGTTTTAGGCCATGAAATATGTGGGGAGATTGTTGGGCTGGGTAAAAATATTGCTGATCTTAAAAATGGTCAGCAAGTTGCTGTGATCCCTTATGTTGCCTGTCAGCAATGCCCGGCGTGTAAAAG  >  NC_000913/4596146‑4596288                                                                           |                                                                     ttttttAGTTATCCACGTGTTTTAGGCCATGAAATATGTGGGGAGATTGTTGGGCTGGGTAAAAATATTGCTGAACTTaaa                                                                <  2:930261/81‑1 (MQ=255)    tttAGTTATCCACGTGTTTTAGGCCATGAAATATGTGGGGAGATTGTTGGGCTGGGTAAAAATATTGCTGAACTTAAAAAt                                                             >  2:930276/1‑81 (MQ=255)        gTTATCCACGTGTTTTAGGCCATGAAATATGTGGGGAGATTGTTGGGCTGGGTAAAAATATTGCTGAACTTAAAAATGGt                                                          >  1:930283/1‑80 (MQ=255)            tCCACGTGTTTTAGGCCATGAAATATGTGGGGAGATTGTTGGGCTGGGTAAAAATATTGCTGAACTTAAAAATGGTCAGc                                                      >  1:930286/1‑80 (MQ=255)               aCGTGTTTTAGGCCATGAAATATGTGGGGAGATTGTTGGGCTGGGTAAAAATATTGCTGAACTTAAAAATGGTCAGCAAGt                                                  >  1:930292/1‑81 (MQ=255)                cGTGTTTTAGGCCATGAAATATGTGGGGAGATTGTTGGGCTGGGTAAAAATATTGCTGAACTTAAAAATGGTCAGCAAGtt                                                 >  2:930280/1‑81 (MQ=255)                    ttttAGGCCATGAAATATGTGGGGAGATTGTTGGGCTGGGTAAAAATATTGCTGAACTTAAAAATGGTCAGCAAGTTGCt                                              <  2:930262/80‑1 (MQ=255)                           ccATGAAATATGTGGGGAGATTGTTGGGCTGGGTAAAAATATTGCTGAACTTAAAAATGGTCAGCAAGTTGCTGTGATccc                                      <  1:930263/81‑1 (MQ=255)                                 aaTATGTGGGGAGATTGTTGGGCTGGGTAAAAATATTGCTGAACTTAAAAATGGTCAGCAAGTTGCTGTGATCCCTTATGt                                >  2:930285/1‑81 (MQ=255)                                                tgttgGGCTGGGTAAAAATATTGCTGAACTTAAAAATGGTCAGCAAGTTGCTGTGATCCCTTATGTTGCCTGTCAGCAATg                 >  1:930295/1‑81 (MQ=255)                                                   tgGGCTGGGTAAAAATATTGCTGAACTTAAAAATGGTCAGCAAGTTGCTGTGGTCCCTTATGTTGCCTGTCAGCAATGccc              >  2:930290/1‑81 (MQ=255)                                                      gCTGGGTAAAAATATTGCTGAACTTAAAAATGGTCAGCAAGTTGCTGTGATCCCTTATGTTGCCTGTCAGCAATGCCCGGc           >  1:930304/1‑81 (MQ=255)                                                          ggTAAAAATATTGCTGAACTTAAAAATGGTCAGCAAGTTGCTGTGATCCCTTATGTTGCCTGTCAGCAATGCCCGGCgtg        >  1:930298/1‑80 (MQ=255)                                                              aaaaTATTGCTGAACTTAAAAATGGTCAGCAAGTTGCTGTGATCCCTTATGTTGCCTGTCAGCAATGCCCGGCGTGTaa     >  2:930284/1‑79 (MQ=255)                                                               aaaTATTGCTGAACTTAAAAATGGTCAGCAAGTTGCTGTGATCCCTTATGTTGCCTGTCAGCAATGCCCGGCGTGTAAAAg  >  1:930288/1‑81 (MQ=255)                                                                           |                                                                     TTTTTTAGTTATCCACGTGTTTTAGGCCATGAAATATGTGGGGAGATTGTTGGGCTGGGTAAAAATATTGCTGATCTTAAAAATGGTCAGCAAGTTGCTGTGATCCCTTATGTTGCCTGTCAGCAATGCCCGGCGTGTAAAAG  >  NC_000913/4596146‑4596288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑