Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	79,341	C→A	G38V (GGT→GTT)	leuD ←	3‑isopropylmalate dehydratase subunit LeuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	79,341	0	C	A	100.0%	144.0 / NA	40	G38V (GGT→GTT)	leuD	3‑isopropylmalate dehydratase subunit LeuD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/13);  total (27/13)

TTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAACCCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAACC  >  NC_000913/79263‑79418                                                                               |                                                                              ttCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAacc                                                                             <  2:18549/81‑1 (MQ=255)  tCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCg                                                                            >  2:18589/1‑81 (MQ=255)    aGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGt                                                                           <  2:18550/80‑1 (MQ=255)    aGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTa                                                                          >  2:18594/1‑81 (MQ=255)        cGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACggg                                                                      <  1:18551/81‑1 (MQ=255)          aaGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGt                                                                     >  1:18587/1‑80 (MQ=255)           aGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCa                                                                   <  2:18552/81‑1 (MQ=255)            gTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCAc                                                                  >  2:18604/1‑81 (MQ=255)            gTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCAc                                                                  >  2:18588/1‑81 (MQ=255)            gTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCAc                                                                  >  1:18611/1‑81 (MQ=255)               cGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACtt                                                                <  2:18553/80‑1 (MQ=255)                   tttGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGc                                                           <  1:18555/81‑1 (MQ=255)                   tttGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGc                                                           <  2:18554/81‑1 (MQ=255)                    ttGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGc                                                           >  1:18608/1‑80 (MQ=255)                     tGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCa                                                          >  1:18593/1‑80 (MQ=255)                          gTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACt                                                    >  1:18584/1‑81 (MQ=255)                           ttGGCCTTTTTCATCCAGAAAACGCCAGTCATTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACt                                                    <  2:18556/80‑1 (MQ=255)                            tGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGt                                                  >  1:18616/1‑81 (MQ=255)                             ggCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGtt                                                 >  2:18607/1‑81 (MQ=255)                                 tttttCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCgg                                             >  1:18586/1‑81 (MQ=255)                                    ttCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGgat                                          >  2:18599/1‑81 (MQ=255)                                      cATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGgatga                                        >  1:18590/1‑81 (MQ=255)                                       aTCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGgatgat                                       >  2:18615/1‑81 (MQ=255)                                          cAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTg                                     <  1:18557/80‑1 (MQ=255)                                          cAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTg                                     >  2:18591/1‑80 (MQ=255)                                          cAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGc                                    <  2:18558/81‑1 (MQ=255)                                           aGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCa                                   >  1:18606/1‑81 (MQ=255)                                            gAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCAt                                  >  1:18623/1‑81 (MQ=255)                                                    cAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATc                           >  2:18596/1‑80 (MQ=255)                                                         gTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACAtt                     <  1:18559/81‑1 (MQ=255)                                                            aaaCAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTggc                  >  1:18614/1‑81 (MQ=255)                                                                 gATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCAt             >  2:18619/1‑81 (MQ=255)                                                                   tGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATc            >  1:18605/1‑80 (MQ=255)                                                                    gcgcgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATc            >  1:18613/1‑79 (MQ=255)                                                                     cgcgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAg         >  1:18603/1‑81 (MQ=255)                                                                     cgcgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAg         >  2:18618/1‑81 (MQ=255)                                                                        gcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCg       >  2:18620/1‑80 (MQ=255)                                                                         ccAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGa     <  1:18560/81‑1 (MQ=255)                                                                           aaaaACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAac   >  2:18630/1‑81 (MQ=255)                                                                           aaaaACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAac   <  1:18562/81‑1 (MQ=255)                                                                           aaaaACCGTACGGGTCACTTTCTACAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAac   <  2:18561/81‑1 (MQ=255)                                                                            aaaaCCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAacc  <  2:18563/81‑1 (MQ=255)                                                                               |                                                                              TTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAACCCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAACC  >  NC_000913/79263‑79418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑