Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	459,044	G→A	V53I (GTC→ATC)	lon →	Lon protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	459,044	0	G	A	100.0%	79.1 / NA	23	V53I (GTC→ATC)	lon	Lon protease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/9);  total (14/9)

ATCCCCTTATTTGTCGGGCGGGAAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATAT  >  NC_000913/458966‑459119                                                                               |                                                                            aTCCCCTTATTTGTCGGGCGGGAAAAATCTATCCGTTGTCTGGAAGCGGTGATGGACCATGATAAAAAAATTATGCTGATc                                                                           >  2:100785/1‑81 (MQ=255) aTCCCCTTATTTGTCGGGCGGGAAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGATc                                                                           >  2:100783/1‑81 (MQ=255)    cccTTATTTGTCGGGCGGGAAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGATcgc                                                                         <  1:100763/80‑1 (MQ=255)         aTTTGTCGGGCGGGAAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGATCGCGCaga                                                                    >  1:100790/1‑80 (MQ=255)          tttGTCGGGCGGGAAAAATCTATCCGTTGTCTGGAAGCTGCGATGGACCATGATAAAAAAATTATGCTGATCGCGCaga                                                                    >  1:100786/1‑79 (MQ=255)          tttGTCGGGCGGGAAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGATCGCGCagaa                                                                   <  2:100764/80‑1 (MQ=255)                gggcgggAAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCt                                                            >  1:100793/1‑81 (MQ=255)                 ggcgggAAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCtt                                                           <  2:100765/81‑1 (MQ=255)                  gcgggAAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCtt                                                           >  2:100791/1‑80 (MQ=255)                     ggAAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCTTCAAc                                                       <  1:143317/81‑1 (MQ=255)                        aaaaTCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCTTCAACGGa                                                    >  2:100802/1‑81 (MQ=255)                                tCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCTTCAACGGATGAGcc                                              <  2:100766/79‑1 (MQ=255)                                      gtttGGAAGCGGCGATGGACCATGATAAAATAATTATGCTGATCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTa                                       >  1:100789/4‑80 (MQ=255)                                             aGCGGCGATGGACCATGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCt                               >  2:100804/1‑81 (MQ=255)                                              gcggcgATGGACCATGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCtt                              >  1:100796/1‑81 (MQ=255)                                                ggcgATGGACCATGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCtttt                            <  1:100767/81‑1 (MQ=255)                                                   gATGGACCATGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCAc                         >  1:100788/1‑81 (MQ=255)                                                      ggACCATGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGt                      >  2:100826/1‑81 (MQ=255)                                                       gACCATGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGt                      <  1:100768/80‑1 (MQ=255)                                                            tGATAAAAAAATTATGCTGATCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGAc                <  1:100769/81‑1 (MQ=255)                                                                aaaaaaaTTATGCTGATCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTg            >  2:100800/1‑81 (MQ=255)                                                                  aaaaaTTATGCTGATCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGc          >  1:100792/1‑81 (MQ=255)                                                                          tGCTGATCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTatat  <  1:100770/81‑1 (MQ=255)                                                                               |                                                                            ATCCCCTTATTTGTCGGGCGGGAAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGAGCCGGGTGTAAACGATCTTTTCACCGTCGGGACCGTGGCCTCTATAT  >  NC_000913/458966‑459119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑