Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	513,613	C→T	G346G (GGC→GGT)	ybaT →	putative transporter YbaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	513,613	0	C	T	100.0%	165.8 / NA	45	G346G (GGC→GGT)	ybaT	putative transporter YbaT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/26);  total (19/26)

TGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGATCCGCCTGCGTCA  >  NC_000913/513539‑513690                                                                           |                                                                              tgGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCAc                                                                           <  2:112460/79‑1 (MQ=255) tgGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCAc                                                                           <  1:112461/79‑1 (MQ=255)    cggcAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGcc                                                                      <  1:112462/81‑1 (MQ=255)    cggcAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGcc                                                                      <  2:112463/81‑1 (MQ=255)     ggcAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGc                                                                       >  2:112503/1‑79 (MQ=255)     ggcAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGc                                                                       >  1:112510/1‑79 (MQ=255)          agTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGtt                                                                <  1:112465/81‑1 (MQ=255)          agTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGtt                                                                <  2:112464/81‑1 (MQ=255)          agTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGtt                                                                >  1:112514/1‑81 (MQ=255)              ccTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGcc                                                             <  2:112468/80‑1 (MQ=255)              ccTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCa                                                            <  1:112467/81‑1 (MQ=255)              ccTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCa                                                            <  2:112466/81‑1 (MQ=255)                tGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAgc                                                          >  2:112533/1‑81 (MQ=255)                 ggggCATCATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAgcg                                                         >  1:112551/1‑81 (MQ=255)                 ggggCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAgcg                                                         >  2:112517/1‑81 (MQ=255)                  gggCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAgc                                                          <  2:112469/79‑1 (MQ=255)                    gCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCa                                                      >  1:112513/1‑81 (MQ=255)                     cAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCAc                                                     <  1:112470/81‑1 (MQ=255)                       aCATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCt                                                   >  1:112534/1‑81 (MQ=255)                          ttattGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCtttt                                                <  1:112471/81‑1 (MQ=255)                             ttGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTg                                              <  1:112473/80‑1 (MQ=255)                             ttGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGa                                             >  1:112519/1‑81 (MQ=255)                             ttGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGa                                             <  1:112472/81‑1 (MQ=255)                               gTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGAtt                                           >  2:112528/1‑81 (MQ=255)                                      tgtTGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTAcc                                    <  1:112474/81‑1 (MQ=255)                                        ttGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCt                                   <  1:112475/80‑1 (MQ=255)                                        ttGATTATGCTGATGACGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTg                                  >  2:112516/1‑81 (MQ=255)                                                   gatgaCGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTtgt                       >  1:112526/1‑81 (MQ=255)                                                    atgaCGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTtgt                       <  1:112476/80‑1 (MQ=255)                                                      gaCGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTgtggt                    >  2:112530/1‑81 (MQ=255)                                                       aCGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTgtggtg                   <  1:112477/81‑1 (MQ=255)                                                       aCGGCGGCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTgtggtg                   >  2:112518/1‑81 (MQ=255)                                                        cggcggCACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGtggtgg                  >  1:112524/1‑81 (MQ=255)                                                        cggcggCACTGAATTTAGGTTCACTCGCCAGCGTAGCCAGCGCCACCTTTTTGATTTGCTACCTAGCAGTGTATGtggtgg                  <  2:112478/81‑1 (MQ=255)                                                         ggcggcACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGc                 >  2:112541/1‑81 (MQ=255)                                                         ggcggcACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGc                 >  2:112520/1‑81 (MQ=255)                                                          gcggcACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCg                <  2:112480/81‑1 (MQ=255)                                                          gcggcACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCg                <  2:112479/81‑1 (MQ=255)                                                              cACTGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGATcc            <  1:112481/81‑1 (MQ=255)                                                                 tGAATTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGATCCGcc         >  1:112523/1‑81 (MQ=255)                                                                   aaTTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGATCCGCCt        <  1:112483/80‑1 (MQ=255)                                                                   aaTTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGATCCGCCTg       <  2:112482/81‑1 (MQ=255)                                                                    aTTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGATCCGCCTGc      <  1:112484/81‑1 (MQ=255)                                                                    aTTTAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGATCCGCCTGc      <  1:112485/81‑1 (MQ=255)                                                                       tAGGTTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGA‑TTGCTACCTTGCGGTGTTTGTGGTGGCGATCCGCCTGCGTCa  >  1:112529/1‑81 (MQ=255)                                                                           |                                                                              TGGCGGCAGAGTACCTGGGGCAACATTATTGTCGTGGTGTTGATTATGCTGATGACGGCGGCACTGAATTTAGGCTCACTCGCCAGCGTTGCCAGCGCCACCTTTTTGATTTGCTACCTGGCGGTGTTTGTGGTGGCGATCCGCCTGCGTCA  >  NC_000913/513539‑513690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑