Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	91,323	G→A	E98K (GAA→AAA)	ftsL →	cell division protein FtsL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	91,323	0	G	A	100.0%	170.0 / NA	46	E98K (GAA→AAA)	ftsL	cell division protein FtsL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/28);  total (18/28)

GGAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATAAGGA  >  NC_000913/91247‑91400                                                                             |                                                                              ggAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAg                                                                           >  2:21226/1‑81 (MQ=255) ggAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAg                                                                           >  2:21231/1‑81 (MQ=255)  gAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGaaaa                                                                            <  1:21177/79‑1 (MQ=255)     cGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATc                                                                       <  1:21178/81‑1 (MQ=255)     cGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATc                                                                       <  1:21180/81‑1 (MQ=255)     cGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATc                                                                       <  1:21179/81‑1 (MQ=255)      gagaTGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCg                                                                      >  1:21232/1‑81 (MQ=255)        gaTGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGcc                                                                    <  2:21181/81‑1 (MQ=255)             tttAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACgg                                                                >  2:21240/1‑80 (MQ=255)               tAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGaa                                                              >  1:21223/1‑80 (MQ=255)               tAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGa                                                               <  2:21182/79‑1 (MQ=255)                    aTTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTg                                                        <  1:21183/81‑1 (MQ=255)                     ttGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTg                                                        >  1:21235/1‑80 (MQ=255)                     ttGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGc                                                       <  2:21184/81‑1 (MQ=255)                     ttGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGc                                                       <  2:21185/81‑1 (MQ=255)                       gAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCa                                                      <  1:21186/80‑1 (MQ=255)                         aTGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAAt                                                   <  1:21187/81‑1 (MQ=255)                              gcAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATgcagc                                              <  2:21188/81‑1 (MQ=255)                                aaCCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCAt                                            >  2:21241/1‑81 (MQ=255)                                  ccTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGt                                          >  1:21249/1‑81 (MQ=255)                                   cTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGtt                                         <  1:21189/81‑1 (MQ=255)                                           tGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGt                                  >  2:21242/1‑80 (MQ=255)                                           tGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGt                                  >  1:21254/1‑80 (MQ=255)                                             aaGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTcac                               <  1:21192/81‑1 (MQ=255)                                             aaGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTcac                               <  1:21190/81‑1 (MQ=255)                                             aaGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTcac                               <  2:21191/81‑1 (MQ=255)                                              agagAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTcaca                              <  1:21193/81‑1 (MQ=255)                                                agaATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTcac                               >  1:21252/1‑78 (MQ=255)                                                 gaATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAg                            >  1:21248/1‑80 (MQ=255)                                                  aaTGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGaa                          <  2:21194/81‑1 (MQ=255)                                                  aaTGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGa                           >  1:21258/1‑80 (MQ=255)                                                   aTGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGaa                          >  2:21228/1‑80 (MQ=255)                                                     gcgcTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGaaaa                        >  1:21237/1‑80 (MQ=255)                                                        cTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATc                    <  1:21196/81‑1 (MQ=255)                                                        cTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATc                    <  1:21195/81‑1 (MQ=255)                                                          cGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCg                   >  2:21246/1‑80 (MQ=255)                                                          cGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCg                   >  2:21250/1‑80 (MQ=255)                                                           ggCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCg                   >  1:21238/1‑79 (MQ=255)                                                           ggCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGt                  <  1:21197/80‑1 (MQ=255)                                                            gCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAg                <  2:21198/81‑1 (MQ=255)                                                              gACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTg              <  1:21199/81‑1 (MQ=255)                                                                ccATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCa            <  1:21200/81‑1 (MQ=255)                                                                 cATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCaa           <  1:21202/81‑1 (MQ=255)                                                                 cATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCaa           <  1:21201/81‑1 (MQ=255)                                                                     gCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAAt       <  1:21203/81‑1 (MQ=255)                                                                      ccGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATa      <  2:21204/81‑1 (MQ=255)                                                                          gTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATAAGGa  <  1:21205/81‑1 (MQ=255)                                                                             |                                                                              GGAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATAAGGA  >  NC_000913/91247‑91400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑