Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	583,611	T→C	intergenic (+551/‑70)	tfaX → / → appY	DLP12 prophage; protein TfaX/DNA‑binding transcriptional activator AppY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	583,611	0	T	C	100.0%	23.6 / NA	8	intergenic (+551/‑70)	tfaX/appY	DLP12 prophage; protein TfaX/DNA‑binding transcriptional activator AppY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/3);  total (5/3)

TTTAATCTTAAATGAAATTTATTAAAATTTGCAAACTATAATTTTGTGTATAAAAATATAAATGCACATCATCCTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCA  >  NC_000913/583533‑583678                                                                               |                                                                    tttAATCTTAAATGAAATTTATTAAAATTTGCAAACTATAATTTTGTGTATAAAAATATAAATGCACATCATCCTGATCAt                                                                   >  2:125643/1‑81 (MQ=255)    aaTCTTAAATGAAATTTATTAAAATTTGCAAACTATAATTTTGTGTATAAAAATATAAATGCACATCATCCTGATCATGAt                                                                >  2:125642/1‑81 (MQ=255)     aTCTTAAATGAAATTTATTAAAATTTGCAAACTATAATTTTGTGTATAAAAATATAAATGCACATCATCCTGATCATGAt                                                                <  1:125633/80‑1 (MQ=255)                           aTTTGCAAACTATAATTTTGTGTATAAAAATATAAATGCACATCATCCTGATCATGATTGTGTATTTAATTGGTTGTTAtt                                         >  1:125644/1‑81 (MQ=255)                                cAAACTATAATTTTGTGTATAAAAATATAAATGCACATCATCCTGATCATGATTGTGTATTTAATTGGTTGTTATTTGact                                    <  2:125634/81‑1 (MQ=255)                                       taATTTTGTGTATAAAAATATAAATGCACATCATCCTGATCATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATc                              >  1:125641/1‑80 (MQ=255)                                                                  acaTCATCCTGATCATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCa  <  1:125635/81‑1 (MQ=255)                                                                   catcatCCTGATCATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGc   >  2:125645/1‑79 (MQ=255)                                                                               |                                                                    TTTAATCTTAAATGAAATTTATTAAAATTTGCAAACTATAATTTTGTGTATAAAAATATAAATGCACATCATCCTGATTATGATTGTGTATTTAATTGGTTGTTATTTGACTACTATCAACTTGTTTTAATTTTATGATAGGTGCA  >  NC_000913/583533‑583678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑