Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	598,242	C→T	A255V (GCC→GTC)	cusB →	copper/silver export system membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	598,242	0	C	T	100.0%	148.2 / NA	40	A255V (GCC→GTC)	cusB	copper/silver export system membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/21);  total (19/21)

GCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAA  >  NC_000913/598166‑598318                                                                             |                                                                             gCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTctg                                                                           <  2:128369/80‑1 (MQ=255)   cAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTctggct                                                                        <  1:128370/81‑1 (MQ=255)    aaaGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTctggctg                                                                       <  1:128371/81‑1 (MQ=255)        aTAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGa                                                                   <  2:128372/81‑1 (MQ=255)         tAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGa                                                                   >  1:128424/1‑80 (MQ=255)          aaCGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGa                                                                   >  2:128417/1‑79 (MQ=255)                gTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGcc                                                           >  1:128426/1‑81 (MQ=255)                    cGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGc                                                       <  2:128373/81‑1 (MQ=255)                       aaaTTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGt                                                    <  1:128374/81‑1 (MQ=255)                        aaTTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGtt                                                   <  2:128375/81‑1 (MQ=255)                           tCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTAc                                                >  1:128411/1‑81 (MQ=255)                           tCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTAc                                                >  2:128422/1‑81 (MQ=255)                            cAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTAc                                                <  2:128376/80‑1 (MQ=255)                             aGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTAccc                                              >  1:128408/1‑81 (MQ=255)                              gggTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCt                                             <  2:128377/81‑1 (MQ=255)                                 tATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCAc                                          >  1:128409/1‑81 (MQ=255)                                   tGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCAcc                                         <  1:128378/80‑1 (MQ=255)                                      aCCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTc                                     <  1:128379/81‑1 (MQ=255)                                       cccGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTcc                                    >  1:128415/1‑81 (MQ=255)                                        ccGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCg                                   <  2:128381/81‑1 (MQ=255)                                        ccGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCg                                   <  2:128380/81‑1 (MQ=255)                                         cGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCgg                                  <  2:128382/81‑1 (MQ=255)                                           gtgtGGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGgcg                                <  1:128383/81‑1 (MQ=255)                                             gtgGGTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGgcgc                               >  1:128436/1‑80 (MQ=255)                                                 gTCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCg                          >  1:128416/1‑81 (MQ=255)                                                  tCACTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCgg                         <  1:128384/81‑1 (MQ=255)                                                    aCTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGa                        >  2:128428/1‑80 (MQ=255)                                                    aCTGCTGCGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGAt                       <  2:128385/81‑1 (MQ=255)                                                        ctgcGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATaaaa                   >  1:128429/1‑81 (MQ=255)                                                         tgcGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATaaaa                   >  1:128420/1‑80 (MQ=255)                                                          gcGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAaca                 >  2:128438/1‑81 (MQ=255)                                                           cGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAacac                >  1:128439/1‑81 (MQ=255)                                                           cGATCCCGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAacac                <  1:128386/81‑1 (MQ=255)                                                                ccGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCAcc           <  1:128387/81‑1 (MQ=255)                                                                 cGGAGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCa          >  1:128451/1‑81 (MQ=255)                                                                    aGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATcc       >  1:128423/1‑81 (MQ=255)                                                                    aGTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATcc       <  2:128388/81‑1 (MQ=255)                                                                     gTCTATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCg      >  1:128433/1‑81 (MQ=255)                                                                        tATCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCa    >  2:128456/1‑80 (MQ=255)                                                                          tCGTCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCaaa  <  2:128389/80‑1 (MQ=255)                                                                             |                                                                             GCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAA  >  NC_000913/598166‑598318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑