Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	612,097	G→A	T133I (ACT→ATT)	fepA ←	ferric enterobactin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	612,097	0	G	A	100.0%	99.1 / NA	28	T133I (ACT→ATT)	fepA	ferric enterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/20);  total (8/20)

GTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAAT  >  NC_000913/612024‑612173                                                                          |                                                                             gTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATcacc                                                                       <  2:131129/81‑1 (MQ=255)  ttGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATcacca                                                                      <  1:131131/81‑1 (MQ=255)  ttGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATcacca                                                                      <  2:131130/81‑1 (MQ=255)    gCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATcaccac                                                                     <  1:131132/80‑1 (MQ=255)      cATAACGCGCAGCTGCCGGACCACGCAGAACATCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACggg                                                                  <  2:131133/81‑1 (MQ=255)            gcgcAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATcgc                                                            <  1:131134/81‑1 (MQ=255)              gcAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCgcgc                                                          >  2:131179/1‑81 (MQ=255)              gcAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCgcgc                                                          <  2:131135/81‑1 (MQ=255)               cAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCt                                                         <  1:131136/81‑1 (MQ=255)                     ccGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTcgccac                                                   <  2:131137/81‑1 (MQ=255)                      cGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTcgccacg                                                  >  2:131180/1‑81 (MQ=255)                           cACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGc                                             >  2:131181/1‑81 (MQ=255)                              gCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCt                                          >  2:131183/1‑81 (MQ=255)                               cAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGTGCTCGCCACGCCAGCCCt                                          >  2:131175/1‑80 (MQ=255)                                 gAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAc                                       <  1:131138/81‑1 (MQ=255)                                  aaCTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACg                                      >  1:131192/1‑81 (MQ=255)                                   aCTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGc                                     <  2:131139/81‑1 (MQ=255)                                         aTACGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAg                               <  1:131140/81‑1 (MQ=255)                                            cGTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAGtt                             <  1:131141/80‑1 (MQ=255)                                             gTTCAATCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAGTTAc                           <  1:131142/81‑1 (MQ=255)                                                 aaTCATTTCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAGTTACGgct                       <  1:131143/81‑1 (MQ=255)                                                     aTTTCAGGTGGCACCCAAGAAATATCACCACGGGTATCGCGCTTGCCACGCCAGCCCTGACGCACCGAGTTACGGCTGCtt                   <  2:131144/81‑1 (MQ=255)                                                       ttCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAGTTACGGCTGCTTAc                 >  2:131182/1‑81 (MQ=255)                                                        tCAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAGTTACGGCTGCTTAcc                >  2:131188/1‑81 (MQ=255)                                                         cAGGTGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAGTTACGGCTGCTTAcc                <  2:131145/80‑1 (MQ=255)                                                             tGGCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGAACCGAGTTACGGCTGCTTACCGGCtt           <  1:131146/81‑1 (MQ=255)                                                               gCACCCAGGAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAGTTACGGCTGCTTACCGGCTTg          <  2:131147/80‑1 (MQ=255)                                                                      ggAAATATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAGTTACGGCTGCTTACCGGCTTGCCGtcaat  <  1:131148/81‑1 (MQ=255)                                                                          |                                                                             GTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAAT  >  NC_000913/612024‑612173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑