Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	102,607	C→T	G125G (GGC→GGT)	ddlB →	D‑alanine‑‑D‑alanine ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	102,607	0	C	T	100.0%	93.0 / NA	26	G125G (GGC→GGT)	ddlB	D‑alanine‑‑D‑alanine ligase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/14);  total (12/14)

AGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTC  >  NC_000913/102530‑102681                                                                              |                                                                           agcaAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCt                                                                          >  1:24121/1‑80 (MQ=255)       cTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGAt                                                                   <  2:24085/81‑1 (MQ=255)       cTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGAt                                                                   <  2:24084/81‑1 (MQ=255)          cTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAg                                                                >  1:24126/1‑81 (MQ=255)               gCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGtt                                                           <  2:24086/81‑1 (MQ=255)                cAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGt                                                            >  1:24132/1‑79 (MQ=255)                  aGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGc                                                        <  1:24087/81‑1 (MQ=255)                   ggTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCa                                                       <  1:24088/81‑1 (MQ=255)                    gTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAg                                                      <  2:24089/81‑1 (MQ=255)                        cGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAAt                                                  <  2:24090/81‑1 (MQ=255)                           tttACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAATTTc                                               >  1:24127/1‑81 (MQ=255)                           tttACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAATTTc                                               <  1:24091/81‑1 (MQ=255)                                 ggTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGctct                                         <  2:24092/81‑1 (MQ=255)                                   tCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTgg                                       >  1:24141/1‑81 (MQ=255)                                     gcgcCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTggg                                      <  1:24093/80‑1 (MQ=255)                                             ggTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGt                             >  2:24179/1‑81 (MQ=255)                                                 gCGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATc                         >  2:24140/1‑81 (MQ=255)                                                  cGTTAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCg                        >  2:24176/1‑81 (MQ=255)                                                    ttAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGtt                      <  2:24094/81‑1 (MQ=255)                                                     tAACCCGCGCAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGtt                      >  2:24136/1‑80 (MQ=255)                                                           gcgcAGAGTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAagccga               <  2:24095/81‑1 (MQ=255)                                                                 agTTTGAAAAAGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCcgcg         <  2:24096/81‑1 (MQ=255)                                                                   tttGAAAAAGGTCTGAGCGATAAGCAGTCAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGaa       <  2:24097/81‑1 (MQ=255)                                                                        aaaaGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTc  >  1:24134/1‑81 (MQ=255)                                                                         aaaGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTc  >  2:24131/1‑80 (MQ=255)                                                                          aaGGTCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTc  >  2:24147/1‑79 (MQ=255)                                                                              |                                                                           AGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTC  >  NC_000913/102530‑102681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑