Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	612,807	A→G	N24S (AAT→AGT)	fes →	enterochelin esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	612,807	0	A	G	96.4%	92.5 / ‑3.7	28	N24S (AAT→AGT)	fes	enterochelin esterase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (19/8);  total (20/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

GTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTAT  >  NC_000913/612735‑612884                                                                         |                                                                              gtgtGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGaaa                                                                       >  2:131296/1‑81 (MQ=255)       cGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTg                                                                 <  1:131269/81‑1 (MQ=255)        ggCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGttt                                                                  >  1:131293/1‑79 (MQ=255)         gCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAg                                                               >  1:131285/1‑81 (MQ=255)          cGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAgg                                                              <  1:131270/81‑1 (MQ=255)           gTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGt                                                             >  1:131294/1‑81 (MQ=255)               aaaGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCAc                                                          <  2:131271/80‑1 (MQ=255)                  gTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTc                                                      >  2:131286/1‑81 (MQ=255)                   tAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCt                                                     <  1:131272/81‑1 (MQ=255)                    aGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCtg                                                    >  2:131299/1‑81 (MQ=255)                         gTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGc                                               >  2:131305/1‑81 (MQ=255)                          tGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCg                                              >  2:131308/1‑81 (MQ=255)                           gagaGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGt                                             >  2:131318/1‑81 (MQ=255)                                cTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATcc                                        >  2:131289/1‑81 (MQ=255)                                  ggtggCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATccc                                       >  1:131291/1‑80 (MQ=255)                                      gCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAgg                                  >  1:131303/1‑81 (MQ=255)                                         gTCGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTc                               >  1:131300/1‑81 (MQ=255)                                           cGAAACATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCt                              >  1:131297/1‑80 (MQ=255)                                             aaaCATGGCCCGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTg                             >  1:131301/1‑79 (MQ=255)                                                      ccGGAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTcg                  <  2:131273/81‑1 (MQ=255)                                                         gAATGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTcgacg               <  1:131274/81‑1 (MQ=255)                                                            tGGCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTCGACGAt             >  1:131309/1‑80 (MQ=255)                                                             ggCAGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTCGACGATa            >  1:131304/1‑80 (MQ=255)                                                                aGCGTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAgc        >  2:131315/1‑81 (MQ=255)                                                                   gTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGcgcg     <  2:131276/81‑1 (MQ=255)                                                                   gTCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGcgcg     <  1:131275/81‑1 (MQ=255)                                                                    tCTGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGcgcg     >  1:131328/1‑80 (MQ=255)                                                                      tGAGTGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTAt  >  1:131341/1‑81 (MQ=255)                                                                         |                                                                              GTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTCGACGATAAAGCGCGTAT  >  NC_000913/612735‑612884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑