Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	619,996	G→A	G68G (GGC→GGT)	fepC ←	ferric enterobactin ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	619,996	0	G	A	100.0%	93.7 / NA	27	G68G (GGC→GGT)	fepC	ferric enterobactin ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/16);  total (11/16)

CGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAATGTGCTCGCCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTAC  >  NC_000913/619921‑620066                                                                            |                                                                       cGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATc                                                                   <  2:132850/81‑1 (MQ=255)    cGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATccag                                                                >  2:132896/1‑81 (MQ=255)     gTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTC‑TTACTGGCGTAATGTTGAATGTGCTCACCATccagc                                                               >  2:132905/1‑80 (MQ=255)           gCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGAc                                                          <  1:132851/80‑1 (MQ=255)                    gcCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCAt                                                  >  1:132898/1‑79 (MQ=255)                           gACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCg                                         <  2:132852/81‑1 (MQ=255)                             ccAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGt                                        >  1:132965/1‑80 (MQ=255)                                   cGGCGTGCAACCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCGTCAgg                                 >  2:132904/1‑81 (MQ=255)                                     gCGTGCAACCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAggcg                               <  1:132853/81‑1 (MQ=255)                                     gCGTGCAACCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAggcg                               <  2:132854/81‑1 (MQ=255)                                       gTGCAACCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAggcggc                             >  2:132952/1‑81 (MQ=255)                                       gTGCAACCTCTTTACTGGCGTAATGTTGAATATGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAggcggc                             <  2:132855/81‑1 (MQ=255)                                          cAACCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTa                          <  1:132856/81‑1 (MQ=255)                                            aCCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAg                        >  1:132908/1‑81 (MQ=255)                                            aCCTCTTTACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAg                        <  1:132857/81‑1 (MQ=255)                                                 tttACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTa                     <  2:132858/79‑1 (MQ=255)                                                   tACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACg                   <  1:132859/79‑1 (MQ=255)                                                   tACTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGc                  <  2:132860/80‑1 (MQ=255)                                                     cTGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAg                <  1:132861/80‑1 (MQ=255)                                                      tGGCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGt               >  2:132914/1‑80 (MQ=255)                                                        gCGTAATGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAAc            <  2:132862/81‑1 (MQ=255)                                                             aTGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGa       <  1:132863/81‑1 (MQ=255)                                                              tGTTGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGAt      <  1:132864/81‑1 (MQ=255)                                                                ttGAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGAttt    <  2:132865/81‑1 (MQ=255)                                                                  gAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTAc  >  2:132913/1‑81 (MQ=255)                                                                  gAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTAc  >  2:132901/1‑81 (MQ=255)                                                                  gAATGTGCTCACCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTAc  >  2:132917/1‑81 (MQ=255)                                                                            |                                                                       CGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAATGTGCTCGCCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTAC  >  NC_000913/619921‑620066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑