Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	662,009	G→T	N90K (AAC→AAA)	lipB ←	lipoyl(octanoyl) transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,009	0	G	T	100.0%	115.0 / NA	32	N90K (AAC→AAA)	lipB	lipoyl(octanoyl) transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/17);  total (15/17)

AGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACC  >  NC_000913/661932‑662084                                                                              |                                                                            aGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGttt                                                                           >  1:141191/1‑80 (MQ=255) aGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGttt                                                                           >  2:141188/1‑80 (MQ=255)  gTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTaa                                                                         <  1:141147/81‑1 (MQ=255)     cAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTATGc                                                                       >  2:141180/1‑80 (MQ=255)     cAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGc                                                                       <  1:141148/80‑1 (MQ=255)     cAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGc                                                                       >  2:141177/1‑80 (MQ=255)      aGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCaa                                                                     >  1:141197/1‑81 (MQ=255)           gggTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACacat                                                                <  2:141149/81‑1 (MQ=255)              tATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACacataca                                                             >  1:141199/1‑81 (MQ=255)               aTTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACacatacat                                                            >  2:141184/1‑81 (MQ=255)               aTTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACacatacat                                                            >  1:141190/1‑81 (MQ=255)                    ccacTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCt                                                       >  2:141198/1‑81 (MQ=255)                      acTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGt                                                     >  2:141200/1‑81 (MQ=255)                         gTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGc                                                  >  1:141185/1‑81 (MQ=255)                             gCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGcccc                                               <  1:141151/80‑1 (MQ=255)                             gCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGcccc                                               <  1:141150/80‑1 (MQ=255)                              cTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCgg                                             >  1:141196/1‑81 (MQ=255)                              cTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCg                                              <  2:141152/80‑1 (MQ=255)                              cTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCg                                              <  2:141153/80‑1 (MQ=255)                                 aagcaagGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGccc                                          <  1:141155/81‑1 (MQ=255)                                 aagcaagGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGccc                                          <  2:141154/81‑1 (MQ=255)                                   gcaagGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGt                                        >  1:141202/1‑81 (MQ=255)                                           aCCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGt                                 >  2:141213/1‑80 (MQ=255)                                           aCCAGTTCACGCACACCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTc                                <  1:141156/81‑1 (MQ=255)                                                        cacCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACcgc                   <  1:141157/81‑1 (MQ=255)                                                        cacCGAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACcgc                   >  2:141208/1‑81 (MQ=255)                                                            gAGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATc               <  1:141158/81‑1 (MQ=255)                                                             aGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCg              <  2:141159/81‑1 (MQ=255)                                                             aGTTTACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCg              >  1:141189/1‑81 (MQ=255)                                                                 tACGGCGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGctct          >  2:732910/1‑81 (MQ=255)                                                                      cGTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATc     <  1:141160/81‑1 (MQ=255)                                                                       gTTTCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCa    <  2:141161/81‑1 (MQ=255)                                                                        tttCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCAc   <  2:141162/81‑1 (MQ=255)                                                                          tCAGTTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCAcc  <  1:141163/80‑1 (MQ=255)                                                                              |                                                                            AGTTCAGCCAGGGTATTCACCACTGTTTGCTCAAGCAAGGTCACCAGTTCACGCACACCGAGTTTACGGCGTTTCAGGTTAAGCAACACATACATCACCTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACC  >  NC_000913/661932‑662084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑