Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	680,675	C→A	A155D (GCT→GAT)	djlC →	co‑chaperone DjlC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	680,675	0	C	A	100.0%	90.8 / NA	26	A155D (GCT→GAT)	djlC	co‑chaperone DjlC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/11);  total (15/11)

TGCTTCTTTGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGCTTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCTTCTGGAATATCCCATTGCGTTG  >  NC_000913/680598‑680745                                                                              |                                                                       tgCTTCTTTGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGAtt                                                                      <  2:144608/80‑1 (MQ=255)   cttcttTGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGAtttt                                                                    <  1:144609/80‑1 (MQ=255)     tcttTGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCtt                                                                 >  1:144652/1‑81 (MQ=255)      cttTGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCtt                                                                 >  1:144641/1‑80 (MQ=255)         tGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTg                                                                <  1:144610/78‑1 (MQ=255)         tGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGa                                                               <  2:144611/79‑1 (MQ=255)           cTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGcc                                                           <  1:144612/81‑1 (MQ=255)                   gCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGc                                                   >  2:144631/1‑81 (MQ=255)                     gTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTg                                                 <  1:144613/81‑1 (MQ=255)                         gAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTg                                              <  1:144614/80‑1 (MQ=255)                         gAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGa                                             >  2:144632/1‑81 (MQ=255)                         gAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGa                                             >  1:144635/1‑81 (MQ=255)                                cattcattTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTc                                      >  1:144639/1‑81 (MQ=255)                                           tGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAg                           >  2:144647/1‑81 (MQ=255)                                             aCAGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGc                          <  2:144615/80‑1 (MQ=255)                                               aGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCtt                       >  1:144646/1‑81 (MQ=255)                                               aGCGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCtt                       >  1:144651/1‑81 (MQ=255)                                                 cGAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCTTCt                     >  1:144654/1‑81 (MQ=255)                                                  gAAGGAATGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCTTCTg                    >  1:144637/1‑81 (MQ=255)                                                       aaTGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCTTCTGGAata               >  1:144645/1‑81 (MQ=255)                                                        aTGGATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCTTCTGGAatat              >  2:144636/1‑81 (MQ=255)                                                           gATGAGGAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCTTCTGGAATATccc           <  2:144616/81‑1 (MQ=255)                                                                ggAAGAAAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCTTCTGGAATATCCCATTGc      >  1:144642/1‑81 (MQ=255)                                                                   agaaAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCTTCTGGAATATCCCATTGCGtt   <  1:144617/81‑1 (MQ=255)                                                                    gaaAGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCTTCTGGAATATCCCATTGCGTTg  <  1:144618/81‑1 (MQ=255)                                                                     aaaGGGAGGATTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCTTCTGGAATATCCCATTGCGTTg  >  2:144649/1‑80 (MQ=255)                                                                              |                                                                       TGCTTCTTTGCTGGCAGAGCGTTTGAACTGGCATTCATTTAATGACAGCGAAGGAATGGATGAGGAAGAAAGGGAGGCTTTTCTTGAGGCCATTCAGGCTGGTGATTGTTTCGATTTCCTTAGCCTTCTGGAATATCCCATTGCGTTG  >  NC_000913/680598‑680745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑